Transcriptomics,Genomics

Dataset Information

21

Microvesicular mRNA profiling of SW480 human colorectal adenocarcinoma cells


ABSTRACT: We analyzed mRNA profile of SW480-derived microvesicles using microarray Keywords: Microvesicular mRNA profiling Overall design: The total RNA was extracted from purified microvesicles derived from SW480 by the single-step isolation method using TRIzol reagent (Invitrogen Corporation, Carlsbad, CA) according to the manufacturer’s protocol. For microarray hybridizations, we followed protocol described in the instruction manual of Illumina TotalPrep RNA Amplification kit (Ambion, Austin, TX). In brief, 640 ng of total RNA from microvesicles was used to synthesize single-stranded cDNA using T7-Oligo(dT) promoter primer and RNase H-facilitated second-strand cDNA, which was purified and served as a template in the subsequent in vitro transcription. The in vitro transcription was carried out in the presence of T7 RNA polymerase and a biotinylated NTP mixture for cRNA. There are four replicate hybridizations. Same amount (Each 1.5 μg) of cRNA was hybridized to arrays, and subsequently labeled with Cy3-streptavidin (Amersham Biosciences, Little Chalfont, UK) and scanned with a Bead Station (Illumina, San Diego, CA).

INSTRUMENT(S): Sentrix Human-6 v2 Expression BeadChip

SUBMITTER: Yong Song Gho  

PROVIDER: GSE9589 | GEO | 2009-12-03

SECONDARY ACCESSION(S): PRJNA103429

REPOSITORIES: GEO

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Publications

Colorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells.

Hong Bok Sil BS   Cho Ji-Hoon JH   Kim Hyunjung H   Choi Eun-Jeong EJ   Rho Sangchul S   Kim Jongmin J   Kim Ji Hyun JH   Choi Dong-Sic DS   Kim Yoon-Keun YK   Hwang Daehee D   Gho Yong Song YS  

BMC genomics 20091125


BACKGROUND: Various cancer cells, including those of colorectal cancer (CRC), release microvesicles (exosomes) into surrounding tissues and peripheral circulation. These microvesicles can mediate communication between cells and affect various tumor-related processes in their target cells. RESULTS: We present potential roles of CRC cell-derived microvesicles in tumor progression via a global comparative microvesicular and cellular transcriptomic analysis of human SW480 CRC cells. We first identif  ...[more]

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