Transcriptomics,Genomics

Dataset Information

20

MicroRNA profile of human medullary thyroid cancer


ABSTRACT: To identify a microRNA profile of human medullary thyroid cancer (MTC), we performed a miRNA microarray analysis exploiting 8 primary tumours and 9 paired neck nodes metastases in comparison with 3 non-neoplastic thyroid tissues. Overall design: Eight sporadic MTC patients were selected for the miRNA microarray analysis. A total of 17 MTC (P = primary tumour, M = nodal metastasis) and 3 non-neoplastic thyroid (N) formalin-fixed paraffin-embedded (FFPE) surgical specimens were obtained. The primary tumours and the matched nodal metastases were obtained from the initial surgery or from a subsequent lymphadenectomy. Referring to the matched primary tumour, 6 of the nodal metastases were synchronous and 3 were metachronous (one patient had both metastasis types included in the array). Non-neoplastic thyroid samples were obtained from patients with pathologies other than thyroid cancer. For each specimen haematoxylin and eosin-stained sections and calcitonin-stained sections were evaluated by an experienced pathologist to confirm the histological diagnosis and to select areas in which tumour cells represented at least the 80% of the total cells.

INSTRUMENT(S): Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (miRNA ID version)

SUBMITTER: Matteo Dugo  

PROVIDER: GSE97070 | GEO | 2018-03-01

REPOSITORIES: GEO

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Publications


This study aimed to identify circulating miRNAs as novel non-invasive biomarkers for prognosis and vandetanib response in advanced medullary thyroid cancer (MTC) patients. We prospectively recruited two independent cohorts of locally advanced/metastatic MTC patients including a subgroup of vandetanib-treated subjects: a discovery cohort (n = 20), including matched plasma/tissue samples (n = 17/20), and a validation cohort, yielding only plasma samples (n = 17). Plasma samples from healthy subjec  ...[more]

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