Transcriptomics,Genomics

Dataset Information

170

Intestinal epithelial and intraepithelial T cell crosstalk mediates a dynamic response to infection


ABSTRACT: Oral infection of mice with Salmonella Typhimurium leads to rapid changes in small intestinal TCRgd IEL behavior. How these changes are induced is currently unknown. By analyzing the transcriptome of TCRgd and intestinal epithelial cells in the steady state and after infection, we find a potential role for epithelial cell MYD88 expression in regulating the IEL response. By additionally analyzing the transcriptome of epithelial cell-specific, conditional Myd88 knockout animals after Salmonella infection, we address the role of epithelial cell Myd88 signaling in regulating the IEL and EC response to Salmonella infection. Overall design: 3 types of mice were used: wildtype C57BL/6 mice, Villin-Cre Myd88ff and Cre negative control mice (C57BL/6 background). Wildtype mice were naïve or orally infected with 10^8 CFU of Salmonella enterica serovar Typhimurium (SL1344) and IELs were harvested from the small intestine after 18 hours. Villin-Cre Myd88ff and Cre negative control mice were treated with Tamoxifen. 1 week after Tamoxifen treatment, mice were orally infected with 10^8 CFU of Salmonella enterica serovar Typhimurium (SL1344) and IELs were harvested from the small intestine after 18 hours.

INSTRUMENT(S): Illumina NextSeq 500 (Mus musculus)

SUBMITTER: Daniel Mucida  

PROVIDER: GSE97184 | GEO | 2017-09-21

SECONDARY ACCESSION(S): PRJNA380919

REPOSITORIES: GEO

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Publications

Intestinal Epithelial and Intraepithelial T Cell Crosstalk Mediates a Dynamic Response to Infection.

Hoytema van Konijnenburg David P DP   Reis Bernardo S BS   Pedicord Virginia A VA   Farache Julia J   Victora Gabriel D GD   Mucida Daniel D  

Cell 20170921 4


Intestinal intraepithelial lymphocytes (IELs) are located at the critical interface between the intestinal lumen, which is chronically exposed to food and microbes, and the core of the body. Using high-resolution microscopy techniques and intersectional genetic tools, we investigated the nature of IEL responses to luminal microbes. We observed that TCRγδ IELs exhibit unique microbiota-dependent location and movement patterns in the epithelial compartment. This behavioral pattern quickly changes  ...[more]

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