Transcriptomics,Genomics

Dataset Information

23

Expression data of colonic epithelial cells colonized with B. theta


ABSTRACT: The gut microbiota is essential for several aspects of host physiology such as metabolism, epithelial barrier function and immunity. Previous studies have revealed that host immune system as well as diet and other environmental factors have a strong impact on the composition and activity of gut microbiota, but the molecular requirements for such functional regulation remain unknown. We show that the bacteria belonging to phylum Bacteroidetes acquire their symbiotic activity in the colonic mucus, depending on a newly characterized molecular family encoded within the polysaccharide utilization loci (PUL), which we have named Mucus-Associated Functional Factor (MAFF). We used microarray analysis of colonic epithlial cells to determin the impact of MAFF genes on colonic homeostasis. Overall design: Specific pathogen free mice were treated with antibiotics (metronidazone and ciprofloxacin) and then colonized wild type or MAFF-deleted mutant of B. theta. Colonic epithelial cells were FACS-purified 4-weeks after colonization, and RNA samples were prepared.

INSTRUMENT(S): [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array

SUBMITTER: Keiichiro Suzuki  

PROVIDER: GSE97504 | GEO | 2018-04-02

REPOSITORIES: GEO

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Publications

IgA regulates the composition and metabolic function of gut microbiota by promoting symbiosis between bacteria.

Nakajima Akira A   Vogelzang Alexis A   Maruya Mikako M   Miyajima Michio M   Murata Megumi M   Son Aoi A   Kuwahara Tomomi T   Tsuruyama Tatsuaki T   Yamada Satoshi S   Matsuura Minoru M   Nakase Hiroshi H   Peterson Daniel A DA   Fagarasan Sidonia S   Suzuki Keiichiro K  

The Journal of experimental medicine 20180724 8


Immunoglobulin A (IgA) promotes health by regulating the composition and function of gut microbiota, but the molecular requirements for such homeostatic IgA function remain unknown. We found that a heavily glycosylated monoclonal IgA recognizing ovalbumin coats Bacteroides thetaiotaomicron (B. theta), a prominent gut symbiont of the phylum Bacteroidetes. In vivo, IgA alters the expression of polysaccharide utilization loci (PUL), including a functionally uncharacterized molecular family provisio  ...[more]

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