Transcriptomics,Genomics

Dataset Information

150

Transcriptome Comparison of phoU Homologies: Genes Deletion strain and the Parent Strain in stationary phase (10 h)


ABSTRACT: Purpose: To compare the transcriptome changes in phoU1(serp0956) or phoU2(serp0316)deletion strain with the parent strain SE1457 in stationary phase (10 h). Methods: Total RNA was isolated and sequenced from the phoU1 deletion strain, phoU2 deletion strain and the parent strain SE1457 in stationary phase (10 h) in triplicate using an illumina high-seq 2500. Raw data was analyzed using TopHat. Genes were considered changed which performed fold-change>=1.5 and FDR<=0.05. Changed genes were then filtered to reveal the downstream targets of phoU1 or phoU2. Results: The expression of 2 genes were significantly decreased and no genes was significantly increased in expression level due to the loss of phoU1 expression.The expression of 279 genes were significantly decreased and 716 genes were significantly increased in expression level due to the loss of phoU2 expression. Overall design: Total three biological samples are analyzied, each of them had triplication.The three samples are parent strain SE1457,the phoU1 deletion strain and phoU2 deletion strain.

INSTRUMENT(S): Illumina HiSeq 2500 (Staphylococcus epidermidis)

SUBMITTER: Xiaofei Wang 

PROVIDER: GSE97656 | GEO | 2017-10-03

SECONDARY ACCESSION(S): PRJNA382568

REPOSITORIES: GEO

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Publications

PhoU2 but Not PhoU1 as an Important Regulator of Biofilm Formation and Tolerance to Multiple Stresses by Participating in Various Fundamental Metabolic Processes in Staphylococcus epidermidis.

Wang Xiaofei X   Han Haiyan H   Lv Zhihui Z   Lin Zhiwei Z   Shang Yongpeng Y   Xu Tao T   Wu Yang Y   Zhang Ying Y   Qu Di D  

Journal of bacteriology 20171114 24


PhoU, a conserved protein that has been proposed to coordinate phosphate import, is a negative regulator of drug tolerance in most bacteria. In Staphylococcus epidermidis, the role of PhoU in biofilm formation and drug tolerance has not yet been investigated. Two PhoU homologs in the genome of S. epidermidis have been identified by the presence of the conserved motif E(D)XXXD of PhoU. We separately constructed ΔphoU1 and ΔphoU2 mutants of S. epidermidis strain 1457. The ΔphoU2 mutant displayed g  ...[more]

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