Transcriptomics,Genomics

Dataset Information

170

Age-dependent comparison of transcriptional signature of lamina propria anti-inflammatory macrophages of Il10rb-/- (KO) and Il10rb+/- (het) mice


ABSTRACT: We report that colonic lamina propria anti-inflammatory macrophages from Il10rb-/- (KO) mice at 12 weeks possessed significantly high levels of proinflammatory transcripts compared to the ones from Il10rb+/- (het) controls. While the transcriptional profile between macrophages from KO and het mice was comparable at 1 weeks and did not possess significant levels of proinflammatory transcripts, we show that the macrophages from KO mice were enriched for proinflammatory transcripts at 3 weeks compared to the ones from 3 week old het controls. These data indicate that the anti-inflammatory signature of colonic lamina propria macrophages from Il10rb-/- mice is compromized at 3 weeks. Overall design: Lamina propria macrophages were FACS sort-purified from 1, 3, and 12 weeks old KO and het mice based on gating strategy; CD45+CD11b+CD11cintCD103-CD64+Ly6C-MHCII+. One mouse each for 3, 12 weeks, and a pool of 3-5 mice at 1 weeks was included in RNA-seq analysis.

INSTRUMENT(S): Illumina NextSeq 500 (Mus musculus)

SUBMITTER: Naresh Singh Redhu  

PROVIDER: GSE97875 | GEO | 2017-08-03

SECONDARY ACCESSION(S): PRJNA383126

REPOSITORIES: GEO

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Publications


Infants with defects in the interleukin 10 receptor (IL10R) develop very early onset inflammatory bowel disease. Whether IL10R regulates lamina propria macrophage function during infant development in mice and whether macrophage-intrinsic IL10R signaling is required to prevent colitis in infancy is unknown. Here we show that although signs of colitis are absent in IL10R-deficient mice during the first two weeks of life, intestinal inflammation and macrophage dysfunction begin during the third we  ...[more]

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