Transcriptomics,Genomics

Dataset Information

89

RNA-Sequencing of 2,4-D treated Brachypodium distachyon roots containing NLS


ABSTRACT: In this study we treated Brachypodium distachyon roots with synthetic auxin, 2,4-D, to induce nodule-like structures (NLS) and performed RNA-seq to assess transcriptome changes during NLS formation. Overall design: Three biological replicates per treatment; total RNA was isolated individually from each replicate.

INSTRUMENT(S): Illumina HiSeq 2500 (Brachypodium distachyon)

SUBMITTER: Arijit Mukherjee  

PROVIDER: GSE97940 | GEO | 2018-04-22

REPOSITORIES: GEO

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Publications

Comparative transcriptome analysis provides key insights into gene expression pattern during the formation of nodule-like structures in Brachypodium.

Thomas Jacklyn J   Bowman Megan J MJ   Vega Andres A   Kim Ha Ram HR   Mukherjee Arijit A  

Functional & integrative genomics 20180306 3


Auxins can induce the formation of nodule-like structures (NLS) in plant roots even in the absence of rhizobia and nitrogen-fixing bacteria can colonize these structures. Interestingly, NLS can be induced in roots of both legumes and non-legumes. However, our understanding of NLS formation in non-legumes at a molecular level is limited. This study aims to investigate NLS formation at a developmental and molecular level in Brachypodium distachyon. We treated Brachypodium roots with the synthetic  ...[more]

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