Transcriptomics,Genomics

Dataset Information

147

MicroRNAs-wide analysis of primordial germ and somatic cells reveals sexual and developmental differential functional regulation in mouse embryonic gonads


ABSTRACT: We have characterized by small-RNAseq the miRNA expression pattern of mouse male and female Primordial Germ Cells (PGCs) and somatic stromal cells from gonads at E11.5, E12.5 and E13.5. MiRNA accumulation was higher in somatic cells than in PGCs and more stable across the different developmental stages analyzed. Differential expression analyses showed differences in the regulation of key miRNA clusters such as miR-199-214, miR-182-183-96 and miR-34c-5p whose targets have defined roles in both germ and somatic cells in gonadal sexual determination. Extensive analyses of miRNA sequences revealed an increase in non-canonical isoforms in PGCs at E12.5 compared to E11.5 and E13.5 and a dramatic change in isomiR expression and non-template 3' nucleotide additions in female PGCs at E13.5 respect to the other samples. Overall design: miRNA profiles of 12 different mice samples, corresponding to PGCs and gonadal somatic cells from E11.5, E12.5 and E13.5 male and female gonads, generated by deep sequencing using MiSeq Sequencing System (Illumina).

INSTRUMENT(S): Illumina MiSeq (Mus musculus)

SUBMITTER: Jesus del Mazo Martinez  

PROVIDER: GSE98713 | GEO | 2018-02-28

REPOSITORIES: GEO

altmetric image

Publications

MicroRNA dynamics at the onset of primordial germ and somatic cell sex differentiation during mouse embryonic gonad development.

Fernández-Pérez Daniel D   Brieño-Enríquez Miguel A MA   Isoler-Alcaraz Javier J   Larriba Eduardo E   Del Mazo Jesús J  

RNA (New York, N.Y.) 20171129 3


In mammals, commitment and specification of germ cell lines involves complex programs that include sex differentiation, control of proliferation, and meiotic initiation. Regulation of these processes is genetically controlled by fine-tuned mechanisms of gene regulation in which microRNAs (miRNAs) are involved. We have characterized, by small-RNA-seq and bioinformatics analyses, the miRNA expression patterns of male and female mouse primordial germ cells (PGCs) and gonadal somatic cells at embryo  ...[more]

Similar Datasets

| GSE74094 | GEO
2013-01-25 | E-GEOD-38126 | ArrayExpress
| GSE79552 | GEO
| GSE97519 | GEO
| GSE40716 | GEO
2012-11-05 | E-GEOD-40716 | ArrayExpress
2012-10-31 | E-GEOD-41908 | ArrayExpress
| GSE76958 | GEO
| GSE76957 | GEO
| GSE76971 | GEO