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Cold adaptation of tRNA nucleotidyltransferases: a tradeoff in activity, stability and fidelity

ABSTRACT: CCA-adding enzymes are highly specific RNA polymerases that synthesize and maintain the sequence CCA at the tRNA 3‘-end. Here, we investigated the impact of cold adaptation on the reactivity and specificity of CCA-adding enzymes from psychrophilic bacteria. A comparative study of the corresponding enzymes from closely related psychro-, meso-, and thermophilic Bacillales indicates that the cold-adapted enzymes show a considerable error rate during CCA synthesis, resulting in additional incorporations of C and A residues. It seems that the activity of psychrophilic CCA-adding enzymes is not only achieved at the expense of structural stability, reaction velocity and substrate affinity, but also results in a reduced polymerization fidelity.

OTHER RELATED OMICS DATASETS IN: PRJNA386487

ORGANISM(S): Geobacillus stearothermophilus Exiguobacterium artemiae Planococcus halocryophilus Bacillus subtilis subsp. subtilis str. 168

PROVIDER: GSE98874 | GEO | 2017/11/03

SECONDARY ACCESSION(S): PRJNA386487

REPOSITORIES: GEO

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