Transcriptomics,Genomics

Dataset Information

48

Campylobacter jejuni genes contributing to acid adaptation


ABSTRACT: In order to cause disease, the food- and water-borne pathogen Campylobacter jejuni must face the extreme acidity of the host stomach as well as cope with pH fluctuations in the intestine. In the present study, C. jejuni NCTC 11168 was grown under mild acidic conditions mimicking those encountered in the intestine. The resulting transcriptional profiles revealed how this bacterium fine-tunes gene expression in response to acid stress. This adaptation involves differential expression of respiratory pathways, induction of genes for phosphate transport and repression of energy generation and intermediary metabolism genes. Keywords: acid shock; dose response; transcriptional response to 3 mild-acidic pH growth conditions (pH6.5, pH 6.0 and pH5.0) Overall design: Three two-condition experiments: (1) pH7.0 versus pH 6.5; (2) pH7.0 versus pH6.0; and (3) pH 7.0 versus pH 5.5. Two biological replicates per experiment and 3 technical replicates for each biological replicate

INSTRUMENT(S): Stintzi Lab Campylobacter jejuni 1.9k array, version 3.0

SUBMITTER: Alain Stintzi  

PROVIDER: GSE9920 | GEO | 2008-01-21

SECONDARY ACCESSION(S): PRJNA105063

REPOSITORIES: GEO

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Publications

Identification of Campylobacter jejuni genes contributing to acid adaptation by transcriptional profiling and genome-wide mutagenesis.

Reid Anne N AN   Pandey Reenu R   Palyada Kiran K   Whitworth Lisa L   Doukhanine Evgueni E   Stintzi Alain A  

Applied and environmental microbiology 20080111 5


In order to cause disease, the food- and waterborne pathogen Campylobacter jejuni must face the extreme acidity of the host stomach as well as cope with pH fluctuations in the intestine. In the present study, C. jejuni NCTC 11168 was grown under mildly acidic conditions mimicking those encountered in the intestine. The resulting transcriptional profiles revealed how this bacterium fine-tunes gene expression in response to acid stress. This adaptation involves the differential expression of respi  ...[more]

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