Genomics

Dataset Information

35

Small RNA sequencing: EVs and the producing cells


ABSTRACT: We report the high-throughput profiling of small RNA in extracellular vesicles (EVs) and their producing mammalian cells using Illumina small RNA sequencing platform. By obtaining over 2190 miRNA expression from 18 samples, the miRNA profile in EVs and their producing cells were compared. Results provide insight into gene profile difference between EV loading and the producing cells. Overall design: Profile of small RNA expression in EVs and the producing cells with small RNA sequencing method.

INSTRUMENT(S): Illumina HiSeq 2500 (Homo sapiens)

SUBMITTER: Thomas Schmittgen  

PROVIDER: GSE99430 | GEO | 2017-05-31

SECONDARY ACCESSION(S): PRJNA388448

REPOSITORIES: GEO

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Publications

Low active loading of cargo into engineered extracellular vesicles results in inefficient miRNA mimic delivery.

Sutaria Dhruvitkumar S DS   Jiang Jinmai J   Elgamal Ola A OA   Pomeroy Steven M SM   Badawi Mohamed M   Zhu Xiaohua X   Pavlovicz Ryan R   Azevedo-Pouly Ana Clara P ACP   Chalmers Jeffrey J   Li Chenglong C   Phelps Mitch A MA   Schmittgen Thomas D TD  

Journal of extracellular vesicles 20170614 1


Extracellular vesicles (EVs) hold great potential as novel systems for nucleic acid delivery due to their natural composition. Our goal was to load EVs with microRNA that are synthesized by the cells that produce the EVs. HEK293T cells were engineered to produce EVs expressing a lysosomal associated membrane, Lamp2a fusion protein. The gene encoding pre-miR-199a was inserted into an artificial intron of the Lamp2a fusion protein. The TAT peptide/HIV-1 transactivation response (TAR) RNA interacti  ...[more]

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