Transcriptomics,Genomics

Dataset Information

162

RNASeq to identify the in vitro molecular signature of Ox40 signaling in conjunction with TCR signaling in CD4 T cells from NZB/W F1 Mice


ABSTRACT: We've recently shown that we can accelerate disease in a model of SLE (the NZB/W F1 model) using an anti-Ox40 mAb treatment regimen. The disease acceleration is rapid (within 2 weeks) but its unclear, mechanistically, how Ox40 promotes disease. To that end we performed RNASeq on in vitro cultured CD4 T cells during Ox40 and TCR stimulation (in a reductionist setting) to understand how Ox40 signaling impacts cellular phenotype and function, including with and without TCR stimulation Overall design: RNASeq was performed on in vitro cultured CD4 T cells from the spleen of NZB/W F1 lupus prone mice, following anti-Ox40 mAb and anti-CD3/CD28 bead stimulation

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Jonathan Sitrin 

PROVIDER: GSE99645 | GEO | 2017-08-18

SECONDARY ACCESSION(S): PRJNA389203

REPOSITORIES: GEO

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Publications

The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice.

Sitrin Jonathan J   Suto Eric E   Wuster Arthur A   Eastham-Anderson Jeffrey J   Kim Jeong M JM   Austin Cary D CD   Lee Wyne P WP   Behrens Timothy W TW  

Journal of immunology (Baltimore, Md. : 1950) 20170710 4


Ox40 ligand (Ox40L) locus genetic variants are associated with the risk for systemic lupus erythematosus (SLE); however, it is unclear how Ox40L contributes to SLE pathogenesis. In this study, we evaluated the contribution of Ox40L and its cognate receptor, Ox40, using in vivo agonist and antagonist approaches in the NZB × NZW (NZB/W) F1 mouse model of SLE. Ox40 was highly expressed on several CD4 Th cell subsets in the spleen and kidney of diseased mice, and expression correlated with disease s  ...[more]

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