Transcriptomics,Genomics

Dataset Information

104

Astrocyte-enriched pan-brain aging transcriptome


ABSTRACT: Normal brain aging is marked by a cognitive decline, spurred by changes in cellular metabolism and homeostatic dysregulation, as well as modifications in synapses and neuronal connectivity. Astrocytes are well positioned as an effector of these changes, although how properties of astrocytes change with age remains unclear. Here, we address this question by profiling astrocytic gene expression from multiple brain regions of adult (4 month-old) and aged (2 years-old) mice. We isolated mRNA from transgenic mice where ribosomes within astrocytes were genetically tagged (GFAP-cre x RPL22-HA, astrocyte ribotag). We then used RNA sequencing to identify and quantify the astrocyte-enriched mRNA, analyzing 4 different brain regions: visual cortex, somatomotor cortex, hypothalamus, and cerebellum. Overall, we find that astrocyte expression of inflammatory and immune response factors increase with age, as well as changes in genes associated with metabolism, cholesterol synthesis, and synaptogenesis. Going forward, these data contribute to an understanding of astrocyte diversity and provide insight into the role of astrocytes in normal aging. Overall design: Profile of aging and adult astrocyte-enriched mRNA from 4 different brain regions from GFAP-Ribotag mice (ribosomal pulldown), with 3 biological replicates and 1 input (non-pulldown) sample per time point and region

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Galina Erikson  

PROVIDER: GSE99791 | GEO | 2017-12-11

SECONDARY ACCESSION(S): PRJNA389601

REPOSITORIES: GEO

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Publications

The Aging Astrocyte Transcriptome from Multiple Regions of the Mouse Brain.

Boisvert Matthew M MM   Erikson Galina A GA   Shokhirev Maxim N MN   Allen Nicola J NJ  

Cell reports 20180101 1


Aging brains undergo cognitive decline, associated with decreased neuronal synapse number and function and altered metabolism. Astrocytes regulate neuronal synapse formation and function in development and adulthood, but whether these properties change during aging, contributing to neuronal dysfunction, is unknown. We addressed this by generating aged and adult astrocyte transcriptomes from multiple mouse brain regions. These data provide a comprehensive RNA-seq database of adult and aged astroc  ...[more]

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