Project description:The purpose of this study was to explore the mechanism of aerobic decay of whole-plant corn silage and the effect of Neolamarckia cadamba essential oil on aerobic stability of whole-plant corn silage. Firstly, the dynamic changes of temperature, microbial community and metabolite content after aerobic exposure of whole-plant corn silage were determined, and the main microbial species and mechanism leading to aerobic spoilage of whole-plant corn silage were analyzed. The N. cadamba essential oil was extracted from fresh N. cadamba leaves by steam distillation, and the minimal inhibitory concentration, antibacterial stability and bacteriostatic mechanism of N. cadamba essential oil against undesirable microorganisms in whole-plant corn silage were determined. According to the minimum inhibitory concentration of N. cadamba essential oil on undesirable microorganisms in silage, N. cadamba essential oil was added to whole-plant corn silage to explore the effect of N. cadamba essential oil on the aerobic stability of whole-plant corn silage.
Project description:Here, we investigated the similarities and differences in the volatile profiles of plants and their holobionts. The VOC profiles of fruits, leaves, stems, and roots from seven plant species, including Turmeric seedlings (Curcuma longa), Artemisia capillaris, Euscaphis japonica, Panax ginseng, Clerodendrum trichotomum, Pyracantha koidzumii, and Japanese cypress (Chamaecyparis obtusa), as well as the VOCs of culturable microbial fractions characterized via GCMS-based metabolomics approaches
Project description:Metarhizium species were grown in plate (M-100 agar) co-culture with corn, for 4 and 7 days. Plate contents (plant material above hypocotyl removed) were ground under liquid nitrogen and extracted with methanol.
Project description:Transcriptional profiling of sweet corn response to plant density (crowding stress). Determine the extent to which hybrid and environment influences crowding stress response and identify crowding stress transcriptional response in sweet corn
Project description:To investigate the effects of corn oil (CO), common drug vehicle, on the gene expression profiles in rat thymus with microarray technique. Female Wistar Rats were administered daily with normal saline (NS), CO 2, 5, 10 ml/kg for 14 days, respectively. Then, the thymus samples of rats were collected for microarray test and histopathology examination. The microarray data showed that 0, 40, 458 differentially expressed genes (DEGs) in 2, 5, 10 ml/kg CO group compared to NS group, respectively. The altered genes were associated with immune response, cellular response to organic cyclic substance, regulation of fatty acid beta-oxidation, et al. However, no obvious histopathologic change was observed in the three CO dosage groups. These data show that 10 ml/kg CO , that dosage has been determined as the vehicle in drug safety assessment , can cause obvious influence on gene expression in rat thymus. Our study suggest that the dosage of CO gavage as the vehicle for water-in-soluble agents in drug development should be no more than 5 ml/kg if agents’ molecular effects in thymus want to be assessed. Gene expression in thymus from female Wistar rats daily administered with 2, 5, 10 ml/kg of corn oil or 10 ml/kg of saline by gavage for 14 consecutive days were measured using Agilent Rat Whole Genome 8×*60K array.
Project description:In the present work, Abortiporus biennis, a white-rot fungus, was studied in regard to its lignocellulolytic enzymatic potential. Secretomics analyses, combined with biochemical methods, were employed to study the enzymatic machinery of the strain, after growth in corn stover cultures and xylose-based defined media. The results revealed the presence of all the necessary enzymatic activities for complete breakdown of the lignocellulosic substrate, while the prominent role of oxidative enzymes in the lignocellulolytic strategy of the strain became evident. Two novel laccases, AbiLac1 and AbiLac2, were isolated from the culture supernatant of this fungus.