Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GNPS_Surferbiome_Morocco

ABSTRACT: Surferbiome metabolomic samples from Morocco less the fecal. LCMS C18 column in positive mode

INSTRUMENT(S): maXis

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: ckapono

PROVIDER: MSV000080444 | GNPS | Tue Jan 03 12:14:00 GMT 2017

REPOSITORIES: GNPS

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Proteomic stable isotope probing (proteomic SIP) using 13C‑E. coli spike‑ins analyzed by DDA LC–MS/MS

Project description:This project applies proteomic stable isotope probing (proteomic SIP) to evaluate detection and quantification of 13C‑labeled E. coli peptides within a complex mouse fecal microbiome background. Proteins were extracted, digested, and quantified before preparing spike‑in mixtures. Mouse fecal peptides (2 µg) were combined with E. coli peptides at 10:1 or 100:1 ratios using defined 13C labeling levels (2%, 5%, 50%), each in triplicate. Peptides were analyzed by nanoLC–MS/MS on an XSelect CSH C18 column coupled to an Orbitrap Fusion Tribrid mass spectrometer in data‑dependent acquisition mode. The dataset enables controlled evaluation of isotopic incorporation, quantitative behavior, and sensitivity of proteomic SIP in microbiome samples.

2026-08-30 | PXD075589 | Pride

GNPS_Surferbiome_Ireland

Project description:Surferbiome Metabolomic samples without fecal samples from Ireland. C18 Positive Mode LCMS

2017-01-03 | MSV000080442 | GNPS

Lee_feline_fecal_12min_gradient

Project description:Fecal samples from 14 different species of felines were extracted using 50:50 MeOH: H2O and chromatographed using a Phenomenex polar C18 column. MS/MS data was acquired on Orbitrap in positive ionization mode on 12 min LC gradient.

2024-02-20 | MSV000094135 | MassIVE

Metabolomic profiles of human fecal samples in different storage conditions

Project description:Data was collected on a LC-MS/MS system (c-18 column) positive mode. Data analyzing storage conditions of different storage collection devices for human fecal samples.

2019-11-07 | MSV000084554 | MassIVE

05092023-pos-pc18-data-backup-JH-human-serum-project

Project description:Human serum samples from Johns Hopkins University. LCMS run on 05-09-2023. Polar C18 column in positive mode. This project is about the chagas disease progression. Four groups in this dataset: infected-progressed, infected-non-progressed, uninfected-progressed, uninfected-non-progressed. We want to compare the progressed and non-progressed in infected groups to see the changes of metabolites related to the disease progression.

2023-08-25 | MSV000092757 | MassIVE

Trapping efficiency of peptides on particle-packed and micro-pillar trap columns for proteomics analyses

Project description:In current work, we compared the separation of HeLa samples using two different trap columns for sample trapping, desalting, and preconcentration. The two columns have different types of the C18 stationary phase: a) a conventional packed PepMap C18 trap-column (Thermo Fischer Scientific), and b) the superficially porous silicon pillars μPAC C18 trap-column (PharmaFluidics)

2026-02-18 | PXD052759 | Pride

Standard Pressure LC-MS/MS Using 1.9um C18 Particles Column at Room Temperature

Project description:Although the benefits of reduction of the size of reversed phase particles are established to provide increased sequencing depth and improved chromatography in LCMS experiments, the wide-scale adoption of optimally sized small particles in reversed-phase columns has been hampered by the necessity for specialized equipment such as ultra-high pressure liquid chromatography or a customized column heating apparatus. Here, we introduce a new strategy to routinely fabricate a 50 cm-long, 1.9 µm particle C18 column and extensively characterize the performance of this column. This column was packed under 100 Bar and routinely utilized on a standard quarternary HPLC at pressures below 300 Bar. Expanding the depth of sequencing of peptides that show a statistically significant quantitative change arising from a biological stimulation is critical. Compared with traditional C18 columns packed with 3 µm particles, the column with the 1.9 µm particles operated with a standard HPLC could detect 330% more peptides with statistically significant changes from differentially stimulated T cells. This improved column fabrication methodology provides an inexpensive improvement for single-run LC-MS/MS analysis to optimize sequencing depth, dynamic range, sensitivity, and reproducibility. This study also highlights the importance of the statistical analysis of quantitative proteomic data instead of a sole focus on peptide spectrum match yields.

2022-02-28 | PXD002895 | Pride

Tjarno Reference Material Dissolved Organic Matter LCMS/MS mass spectra

Project description:Marine Dissolved Organic Matter analysed by reversed phase LCMS on C18 with data dependent analysis, positive and negative mode.

| MSV000090282 | MassIVE

NISTfecalRM_Astral_HypersilGoldC18_positive

Project description:NIST fecal reference material extracted with 50% MeOH and prepared in different dilutions and reconstitution solvents. The analysis was conducted using a Hypersil Gold (C18) in positive ionization mode on a Astral via different MS-modes (positive ionization mode).

2023-11-28 | MSV000093526 | MassIVE

Metabolome of the Medicago truncatula symbiosome space, bacteroid, and nodule cytosol

Project description:This study characterizes the metabolome of the Medicago truncatula-Sinorhizobium meliloti symbiosis. Symbiosome space, bacteroid, and nodule cytosol fractions were isolated from 4-week-old root nodules and profiled by untargeted liquid chromatography-tandem mass spectrometry (LC-MS/MS). Polar metabolites were separated on a BEH Amide column and non-polar metabolites on a reversed-phase C18 column, each acquired in positive and negative ionization modes. Three biological replicates were analyzed per sample type, together with pooled quality-control samples. This dataset accompanies our proteomic and metabolomic profiling of the symbiosome and supports the finding that the symbiosome space is an active metabolic compartment enriched in soluble sugars and amino acids.

2026-08-19 | MTBLS15392 | MetaboLights

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data