Project description:Serum of LCMV infected mice. Data was generated on a Thermo Q Exactive and C18 RP UHPLC. Positive polarity acquisition on LC-MS/MS.
Project description:Investigation of small molecules detected in bacterial inhibitory zone. Data were generated on a Thermo Q Exactive and C18 RP UHPLC. Positive polarity acquisition on LC-MS/MS.
Project description:Genome-wide mapping of gene-microbiome interaction: implication in behavior and effect on microbiome and metabolome. Data was generated on a Thermo Q Exactive and C18 RP UHPLC. Positive polarity acquisition on LC-MS/MS.
Project description:Small molecule analysis of cheese culture extracts, standard methanol extraction; iron limitation and additions. Data were acquired using a Thermo Q Exactive and C18 RP-UHPLC. Positive polarity acquisition of LC-MS/MS.
Project description:Metabolic study of various Bile acids. Data was generated on a Thermo Q Exactive and C18 RP UHPLC. Positive polarity acquisition on LC-MS/MS.
Project description:Metabolic study of various Standards. Data was generated on a Thermo Q Exactive and C18 RP UHPLC. Positive and Negative polarity acquisition on LC-MS/MS.
Project description:<p _msttexthash='64160037318' _msthash='272'>Ultra-high-performance liquid chromatography (UHPLC) was conducted using a Vanquish UHPLC system (Thermo Fisher), coupled with a Q Exactive™ HF/Q Exactive™ HF-X mass spectrometer for liquid chromatography-mass spectrometry (LC-MS) analysis. To ensure high resolution and effective analyte retention, a Hypesil Gold chromatographic column (100 × 2.1 mm, 1.9 µm) was used to separate the samples by hydrophilic interaction chromatography (HILIC). In both positive and negative electrospray ionization (ESI) modes, the chromatographic parameters were as follows: flow rate: 0.2 mL/min, column temperature: 40℃, mobile phase A = 0.1% formic acid, B = methanol. The separated metabolites were then analyzed via mass spectrometry, with a scanning range of m/z 100–1500. The MS/MS secondary scan was performed using a data-dependent acquisition mode, and QC samples were used to correct instrument drift and signal fluctuation. Subsequently, the raw LC-MS data files were processed using Compound Discoverer 3.3 (CD3.3; Thermo Fisher Scientific) (Cooper and Yang, 2024). Initially, each metabolite was screened, and the peak area was normalized using the first QC sample. In the subsequent steps, high-resolution molecular ion peaks and characteristic fragment ions were used to predict molecular formulas, and compared with the mzCloud, mzVault, and MassList databases to achieve accurate metabolite identification and relative quantification.</p>
Project description:Metabolic analysis on rats on the effect of circadian disruption. Data was generated on a Thermo Q Exactive and C18 RP UHPLC. Positive acquisition of LC-MS/MS
Project description:Metabolic study on the effect of sleep disruption on mice. Data was generated on a Thermo Q Exactive and C18 RP UHPLC. Positive polarity acquisition on LC-MS/MS.