ABSTRACT: Untargeted metabolomics of mouse intestinal and serum tissue
from adult mice infected with LCMV. Data was acquired using a Thermo Q-Exactive and C18 RP-UHPLC.
Project description:Untargetted metabolomics of mouse vaginal, cervix, and uterine tissue from adult mice colonized with GBS, candida or trichomonas. Data was acquired using a Bruker Daltronics maXis Impact and a C18 RP-UHPLC system. Positive polarity acquisition of LC-MS/MS
Project description:To understand the impact of murine rotavirus infection on mouse intestinal epithelial tissue, we isolated total intestinal epithelium from uninfected and infected C57Bl6J mice and performed single-cell RNAseq.
Project description:mRNA expression data from BALB/c mice which were infected intranasally with Respiratory Syncytial Virus (or Hep-2 cell lysate control) at 1 week old and challenged with PBS or house dust mite (HDM) extract as adults. Experimental groups: RH – neonatal RSV, adult HDM, RP – neonatal RSV, adult PBS, HH – neonatal Hep-2, adult HDM and HP – neonatal Hep-2, adult PBS.
Project description:Serum of LCMV infected mice. Data was generated on a Thermo Q Exactive and C18 RP UHPLC. Positive polarity acquisition on LC-MS/MS.
Project description:Background. Pneumocystis jirovecii pneumonia (PCP) is a leading cause of fungal pneumonia, but its diagnosis primarily relies on invasive bronchoalveolar lavage (BAL) specimens that are difficult to obtain. Oropharyngeal swabs and serum could improve the PCP diagnostic workflow, and we hypothesized that CRISPR could enhance assay sensitivity to allow robust P. jirovecii diagnosis using swabs and serum. Herein we describe the development of an ultrasensitive RT-PCR-coupled CRISPR assay with high active-infection specificity in infant swabs and adult BAL and serum. Methods. Mouse analyses employed an RT-PCR CRISPR assay to analyze P. murina transcripts in wild-type and Rag2-/- mouse lung RNA, BAL, and serum at 2-, 4-, and 6-weeks post-infection. Human studies used an optimized RT-PCR CRISPR assay to detect P. jirovecii transcripts in infant oropharyngeal swab samples, adult serum, and adult BAL specimens from P. jirovecii-infected and P. jirovecii-non-infected patients. Results. The P. murina assays sensitively detected Pneumocystis RNA in the serum of infected mice throughout infection. Oropharyngeal swab CRISPR assay results identified infants infected with P. jirovecii with greater sensitivity (96.3% vs. 66.7%) and specificity (100% vs. 90.6%) than RT-qPCR compared to mtLSU standard marker, and CRISPR results achieved higher sensitivity than RT-qPCR results (93.3% vs. 26.7%) in adult serum specimens. Conclusion. Since swabs are routinely collected in pediatric pneumonia patients, serum is easier to obtain than BAL, and RT-PCR CRISPR results may not detect P. jirovecii colonization, this assay approach could improve pediatric Pneumocystis diagnosis by achieving specificity for active infection and avoiding the requirement for BAL specimens.
Project description:Roundworm infections result in morbidity, causing significant health and economic concerns in humans and pigs, respectively. We investigated the humoral responses of A. suum infected pigs before and after transition from larval to adult stage and confirmed our previous report on the diagnostic value of human Ascaris-specific antibodies. We evaluated the systemic and mucosal humoral responses in Ascaris infected pigs at 14- and 35-days post-infection (dpi). Ascaris-specific antibodies against larval and adult worm antigens and adult excretory/secretory (ES) products in serum, broncho-alveolar lavage fluid and intestinal mucus were quantified by ELISA. IgA+ B cells in jejunal/ileal mesenteric lymph nodes (mLNs) were investigated using flow cytometry.
Project description:Fecal samples were collected from Trypanosoma cruzi-infected (strain CL+Luc) and uninfected mice for up to 3 months post-infection. Samples were extracted with 50% methanol.
Project description:Systemic sclerosis (SSc) is a complex autoimmune disease characterized by microvascular dysfunction, immune dysregulation, and progressive fibrosis, and can be classified into limited cutaneous (lcSSc) and diffuse cutaneous (dcSSc) forms. Primary Raynaud’s phenomenon (RP) is a functional vascular disorder that may precede or occur independently of connective tissue diseases. Circulating microRNAs (miRNAs) have emerged as regulators of vascular and immune processes and can be detected in serum. In this study, serum miRNA profiles were assessed in patients with SSc, including lcSSc and dcSSc subtypes, RP, and healthy controls. Small RNA sequencing was performed in a discovery cohort to characterize circulating miRNA expression across the study groups. Libraries were prepared from serum-derived RNA and sequenced on an Illumina platform. Raw sequencing data were processed using a standardized pipeline for miRNA quantification, and normalized counts were generated for downstream analyses. This dataset provides a resource for the exploration of circulating miRNA profiles in SSc subtypes and RP.
Project description:Transcriptional profiling of intestinal response to Citrobacter rodentium in wild-type and Nlrp12-deficient mice Four-conditions experiment, Nlrp12-deficient mouse infected by Citrobacter rodentium at day 7 versus non-infected Nlrp12-deficient mice with two biologicals replicates , Wild-type mouse infected by Citrobacter rodentium at day 7 versus non-infected Wild-type mice with two biologicals replicates and Nlrp12-deficient mouse infected by Citrobacter rodentium versus Control mouse infected by Citrobacter rodentium at 2 differents times ( day 0 and post infection at day 7 ) with three biologicals replicates