Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GNPS Schkuhria

ABSTRACT: Dichloromethane crude extract from Schkuhria pinnata and fractions

INSTRUMENT(S): LCMS-IT-TOF

ORGANISM(S): Schkuhria Pinnata (ncbitaxon:176579)

SUBMITTER: Mark Kimani

PROVIDER: MSV000081834 | GNPS | Wed Dec 20 06:52:00 GMT 2017

REPOSITORIES: GNPS

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA-seq analysis of differentially expressed genes between stromal vascular fraction (SVF) from inguinal white adipose tissue (iWAT) of mice treated with medicinal herb extract (MHE) and Phosphate-buffered saline (PBS)

Project description:To assess the influences of a crude medicinal herb extract (MHE) on the immune composition and function in inguinal white adipose tissue (iWAT), we isolated iWAT stromal vascular fractions (SVFs) from control (PBS) and MHE-treated mice and performed RNA-seq analysis. iWAT SVF of mice treated with MHE was defined as the treatment group. Phosphate-buffered saline (PBS) treatment was used as the control group. Then RNA-Seq experiment was performed by OE Biotech Co., Ltd. (Shanghai, China) to analyze gene expression changes in iWAT SVF.

2024-10-09 | GSE279130 | GEO

Label free nano-LC MALDI-TOF-MS/MS analysis of Triton X-100 solubilized proteins from bovine brain capillary endothelial cells (BCECs).

Project description:Triton X-100 soluble crude extract from BCECs displaying limited blood-brain barrier functions was fractionated into 5 fractions (F0, F25, F50, F75) with increasing concentrations of acetonitrile (0%, 25%, 50%, 75%). This is the merging of triplicate dataset of subfraction # F0.

2012-11-27 | PRD000681 | Pride

Anti-cancer activity of Cotyledon orbiculata extract

Project description:We investigated the effects of the crude extract of a South African medicinal plant, Cotyledon orbiculata, on cell survival of colon (HCT116) cancer cell lines. Using RNASeq, we discovered that the extract interfered with mRNA regulatory pathways. Here, we found that the extract of Cotyledon orbiculata, a South African medicinal plant, had an anti-proliferative effect in cancer cells, mediated by apoptosis induced by alternative splicing of hnRNPA2B1 and BCL2L1.

2020-10-06 | GSE156221 | GEO

Fluidigm Biomark 96.96 nanofluidic arrays for qRT-PCR of 93 miRNAs: salivary extracellular vesicles

2016-08-01 | GSE78763 | GEO

Fluidigm Biomark 96.96 nanofluidic arrays for qRT-PCR of 93 miRNAs: salivary extracellular vesicles

Project description:Crude extracellular vesicles (EVs) from eight healthy volunteers were separated into 6 fractions based on their densities by using the iodixanol-based density gradient centrifugation method. To determine the distribution of miRNAs among these fractions, quantities of 93 miRNAs were quantified by the TaqMan real time PCR method using the BioMark HD system (Fluidigm) equipped with 96.96 dynamic array (Fluidigm). Six samples were fractionated from a crude EVs by density gradient centrifugation. Total of 48 samples were prepared from 8 healthy volunteers. Technical replicate of 4 gave 8 x 6 x 4 x 93 = 17,856 data. As control Tris-HCl EDTA buffer (TE) was used.

2016-08-01 | E-GEOD-78763 | biostudies-arrayexpress

MRMaid - crude total soluble protein extract from Arabidopsis thaliana

Project description:crude total soluble protein extract from Arabidopsis thaliana

2012-06-11 | PRD000717 | Pride

GNPS - Pisolithus arhizus fruiting bodies dichloromethane methanol extract

Project description:dichloromethane-Methanol (1:1) extract and fractions obtained from fruiting bodies of Pisolithus arhizus

2025-07-28 | MSV000098659 | GNPS

Functional Genomics Screen with Pooled shRNA Library and Gene Expression Profiling with Extracts of Azadirachta indica Identify Potential Pathways for Therapeutic Targets in Head and Neck Squamous Cell Carcinoma

Project description:We investigate the molecular targets and pathways that the neem extracts and the associated compounds act through, to bring about tumor suppression by using a genome-wide functional pooled shRNA screen on head and neck squamous cell carcinoma cell line treated with crude neem leaf extracts. We analyzed differences in global clonal sizes of the shRNA-infected cells cultured under no treatment and treatment with neem leaf extract conditions, assayed using next-generation sequencing. We further analyzed differences in gene expression in HSC-4 cells, upon treatment with neem leaf extract in a time-dependent manner, followed by a time-dependent rescue. Our results indicate that neem extract simultaneously affects various important molecular signaling pathways in head and neck cancer cells, some (TGF-β/HSF-1) of which may be therapeutic targets for this devastating tumor.

2019-01-15 | GSE125021 | GEO

Vietnamese medicinal plant Melicope pteleifolia leaves

Project description:Crude CHCl3-MeOH extract and n-hexane, CHCl3, and EtOAc fractions from leaves of Melicope pteleifolia collected in Vietnam. Subfractions obtained from n-hexane fraction are also included.

2022-02-02 | MSV000088768 | MassIVE

PAPE - Pre-fractionation assisted phosphoprotein enrichment, a novel approach for phosphoproteomic analysis of green tissues from plants

Project description:Biological replicates: 393-395 (crude extract): protein extraction 399-401 (40% AS): protein extraction+40% AS precipitation 405-407 (crude extract+MOAC): protein extraction+phosphoprotein enrichment by MOAC 408-410 (40% AS+MOAC): protein extraction+40% AS precipitation+phosphoprotein enrichment by MOAC -determination of protein concentration by 2D-Quant Kit -LC-MS with LTQ Orbitrap Velos: nanoLC, precursor scan Orbitrap, fragmentation CID, fragment scan LIT -Thermo Proteome Discoverer 1.3 with phosphoRS 1.0 -Mascot 2.3 -database TAIR 10 -Scaffold 4 Technical replicates two measurements each (e.g. 393, 393_2)

2014-01-28 | PXD000421 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data