Project description:Skin samples collected from underarm w/ PDMS for 30 seconds. Samples used for optimization of GC headspace methodology wrt desorption time, cryofocusing, and size of PDMS patch (10mL vials were used).
Project description:GC data for three volunteers. The skin across several body sites was sampled with PDMS patch overnight. The volatiles were desorbed in vial at 200 C.
Project description:The skin of three volunteers has been sampled with PDMS patches across various body sites. The volatiles were desorbed at 200C and analyzed with GC-MS.
Project description:The skin of three volunteers has been sampled with PDMS patches across various body sites. The volatiles were desorbed at 200C and analyzed with GC-MS. Re-submission with mzML files
Project description:Skin samples collected from underarm w/ PDMS for 30 seconds. Samples used for optimization of GC headspace methodology wrt desorption time, cryofocusing, and size of PDMS patch (10mL vials were used).
Project description:Dataset from a shipboard incubation experiment of an ocean surface-water microbial community sampled at 25m depth at Station ALOHA in the North Pacific Subtropical Gyre. Incubations were amended with ammonium, glutamate, leucine, nitrate and urea, in two isotopic variants: 15N (to track incorporation by various community members) and 14N (for quantitation of abundance changes by diDO-IPTL).
Project description:Marine microbial communities are critical for biogeochemical cycles and the productivity of ocean ecosystems. Primary productivity, at the base of marine food webs, is constrained by nutrient availability in the surface ocean, and nutrient advection from deeper waters can fuel photosynthesis. In this study, we compared the transcriptional responses by surface microbial communities after experimental deep water mixing to the transcriptional patterns of in situ microbial communities collected with high-resolution automated sampling during a bloom in the North Pacific Subtropical Gyre. Transcriptional responses were assayed with the MicroTOOLs (Microbiological Targets for Ocean Observing Laboratories) marine environmental microarray, which targets all three domains of life and viruses. The experiments showed that mixing of deep and surface waters substantially affects the transcription of photosystem and nutrient response genes among photosynthetic taxa within 24 hours, and that there are specific responses associated with the addition of deep water containing particles (organisms and detritus) compared to filtered deep water. In situ gene transcription was most similar to that in surface water experiments with deep water additions, showing that in situ populations were affected by mixing of nutrients at the six sampling sites. Together, these results show the value of targeted metatranscriptomes for assessing the physiological status of complex microbial communities.
Project description:All samples were collected on cotton then placed into 2 mL borosilicate vials. The GC MS analysis was carried out using the Agilent 7200 GC QTOF Agilent Technologies Santa Clara CA. equipped with a robotic sampler system. The separation was conducted on an HP 5MS column (30 m x 0.25 mm x 0.25 micro m). The patch within the vial was heated for 25 min. at 200 C to desorb volatiles from the patch and 0.5 mL of headspace injected (injector temperature set at 250 C) into the instrument with a headspace syringe heated to 145 C. The GC protocol analysis included starting temperature 45 C min oven ramp (hold of 2 min), 15 C per min oven ramp to 300 C (hold of 3 min), and 50 C per min oven ramp to 320 C purge the column. The helium carrier gas was set to constant 1.2 mL per min flow and a spitless injection mode was applied. The purge flow to split vent rate was 50 ml per min at 1 min. The scanned m z range was 35-400 with the acquisition rate of 10 spectra per s. The empty vial blanks were interspersed with the samples to assess the background signal.