GNPS - Pseudomonas psychrophila strain JB418 W and M
Ontology highlight
ABSTRACT: Bacterial SPE extracts. Untargeted mass spectrometry data were acquired using a Thermo Q Exactive and C18 RP UHPLC. Positive polarity acquisition of LC-MS/MS.
Project description:Investigation of small molecules detected in bacterial inhibitory zone. Data were generated on a Thermo Q Exactive and C18 RP UHPLC. Positive polarity acquisition on LC-MS/MS.
Project description:Serum of LCMV infected mice. Data was generated on a Thermo Q Exactive and C18 RP UHPLC. Positive polarity acquisition on LC-MS/MS.
Project description:Samples of cotton leaves, fall armyworm midgut content and faeces. Data was generated on a Thermo Q Exactive and C18 RP UPLC. Positive polarity acquisition on LC-MS/MS
Project description:Samples of cotton leaves, fall armyworm midgut content and faeces. Data was generated on a Thermo Q Exactive and C18 RP UPLC. Negative polarity acquisition on LC-MS/MS
Project description:The dataset contains the data of key experiments for developing a novel and highly sensitive bottom-up phosphoproteomics strategy termed ERLIC-SCX/RP-LC-MS. We compared two SPE types (RP, SCX) for an ERLIC run with 7 fractions. The results were used to design a workflow for highly sensitive bottom-up phosphoproteomics (third experiment) combing ERLIC in 21 fractions with the fractions 1-9 cleaned by SCX-SPE and the fractions 10-21 by RP-SPE. Up to 50% of the fractions were subjected to LC-MS/MS analysis with 45 h of total MS time per replicate.
Project description:Small molecule analysis of cheese culture extracts, standard methanol extraction; iron limitation and additions. Data were acquired using a Thermo Q Exactive and C18 RP-UHPLC. Positive polarity acquisition of LC-MS/MS.
Project description:Genome-wide mapping of gene-microbiome interaction: implication in behavior and effect on microbiome and metabolome. Data was generated on a Thermo Q Exactive and C18 RP UHPLC. Positive polarity acquisition on LC-MS/MS.
Project description:Proteome analysis of bacterial strain Thermodesulfobium acidiphilum 3127-1 was done using off-line SCX fractionation of tryptic peptides with subsequent LC-MS/MS analyses of obtained fractions using Q Exactive HF mass spectrometer.
Project description:Investigation of small molecules detected in bacterial inhibitory zone. Data were generated on a Thermo Q Exactive and C18 RP UHPLC. Positive polarity acquisition on LC-MS/MS.
Project description:A ChIP-seq assay was performed to identify the regulons of an ompR-like transcription factor (gene name: 13375, GenBank: AYL80818.1) in Pseudomonas syringae pv. actinidiae. An 13375-overexpressing mutant G1-OE13375, which constitutively express C-terminally Myc-tagged ompR-like gene in the 13375-deletion mutant G1Δ13375, was used in this study. The bacterial cells were cultured either in nutrition-rich KB medium or hrp-derepressing medium (HDM) at 25 C for 24 hours. A PierceTM Magnetic ChIP Kit (Cat. #: 26157, Thermo Fisher Scientific) and a ChIP-grade Myc-Tag Monoclonal Antibody (Myc.A7, Cat. #: MA121316, Thermo Fisher Scientific) were used for sample pretreatment and immunoprecipitation.