Project description:Top-down proteomics of venom protein of venom-gland organoids aspidelaps. Samples were extracted with MilliQ water and proteins reduced with TCEP before top-down LC-MS/MS analysis.
Project description:Top-down proteomics of venom protein of venom-gland organoids aspidelaps. Samples were extracted with MilliQ water and proteins reduced with TCEP before top-down LC-MS/MS analysis.
Run 2
Project description:Venom proteomics analysis of Walterinnesia aegyptia and Walterinnesia morgani. For top-down analysis, venom samples were reduced with TCEP and measured via HPLC-MS/MS (Q-Exactive and LTQ-Orbitrap XL).
Project description:Sea nettle jellyfish cause millions of painful stings annually with little known about how their venom works and no rational treatments available. Here we perform a systematic analysis of sea nettle venom / host interactions. The venom shows dose-dependent cytotoxic activity in human cells and this can be blocked by dual inhibition of apoptosis and necroptosis. Using whole-genome CRISPR screening we identified human genes and pathways that modify venom action. The top gene cluster identified regulates proteoglycan biosynthesis. We show exogenous heparin, a drug used clinically as an anticoagulant, blocks venom cytotoxicity at a physiologically relevant dose. This effect was therapeutic, inhibiting venom even one hour after exposure. Importantly, in vivo, heparin protected against acute spontaneous pain, thermal hyperalgesia, and mechanical allodynia induced by venom. This provides the exciting possibility of repurposing heparin, a safe, commercially available drug as a prophylactic or therapeutic to reduce the impact of sea nettle stings.
Project description:Top-down and bottom-up protein analysis of venom and saliva of solenodon. Venom and saliva was separated by HPLC and either directly analysis by HR FT MS/MS (Top-down) or further decomplexed by SDS-PAGE followed by in-gel trypsine digestion and HR LC-MS/MS analysis (bottom-up, Venom only). For shotgun bottom-up comparison of venom and saliva proteins, samples were directly reduced, alkylated, digested with trypsin and measured by HPLC-MS/MS. Additional bottom-up analysis was performed from bioactivity guided fractionation experiments.
Project description:Top-down Proteomics of Venom from Naja haje from the Berlin Zoo. 4 technical replicates from TCEP reduced crude venom and 1 replicate from native venom.
Project description:Genus-wide proteomics analysis of cobra (Naja) venoms. For top-down analysis, venom samples were reduced with TCEP and measured via HPLC-MS/MS (Q-Exactive) . Spectrum-Protein matching was performed with TopPic1.1 against a genus wide translated transcriptome database and NCBI protein entries from the najas.
For bottom-up analysis, HPLC separated fraction of venom were dried down, reduced with triethylphosphine and alkylated with 2-iodoethanol. HPLC-MS/MS experiments were performed on normalflow UHPLC-QTOF system (AB Sciex 5600 TripleTOF). Data analysis (PSM) was performed ProteinPilot version 4.0 and the transcriptome derived protein sequence database.