Genus wide venom proteomics of cobras (Naja)

ABSTRACT: Genus-wide proteomics analysis of cobra (Naja) venoms. For top-down analysis, venom samples were reduced with TCEP and measured via HPLC-MS/MS (Q-Exactive) . Spectrum-Protein matching was performed with TopPic1.1 against a genus wide translated transcriptome database and NCBI protein entries from the najas. For bottom-up analysis, HPLC separated fraction of venom were dried down, reduced with triethylphosphine and alkylated with 2-iodoethanol. HPLC-MS/MS experiments were performed on normalflow UHPLC-QTOF system (AB Sciex 5600 TripleTOF). Data analysis (PSM) was performed ProteinPilot version 4.0 and the transcriptome derived protein sequence database.

INSTRUMENT(S): Q-Exactive, TripleTOF 5600

ORGANISM(S): Naja (ncbitaxon:8638)

SUBMITTER: Eivind Undheim   Daniel Petras  

PROVIDER: MSV000081885 | MassIVE | Thu Jan 04 17:11:00 GMT 2018

SECONDARY ACCESSION(S): PXD008597

REPOSITORIES: MassIVE