Proteomics

Dataset Information

0

Genus wide venom proteomics of cobras (Naja)

ABSTRACT: Genus-wide proteomics analysis of cobra (Naja) venoms. For top-down analysis, venom samples were reduced with TCEP and measured via HPLC-MS/MS (Q-Exactive) . Spectrum-Protein matching was performed with TopPic1.1 against a genus wide translated transcriptome database and NCBI protein entries from the najas. For bottom-up analysis, HPLC separated fraction of venom were dried down, reduced with triethylphosphine and alkylated with 2-iodoethanol. HPLC-MS/MS experiments were performed on normalflow UHPLC-QTOF system (AB Sciex 5600 TripleTOF). Data analysis (PSM) was performed ProteinPilot version 4.0 and the transcriptome derived protein sequence database.

INSTRUMENT(S): Q-Exactive, TripleTOF 5600

ORGANISM(S): Naja (ncbitaxon:8638)

SUBMITTER: Eivind Undheim   Daniel Petras  

PROVIDER: MSV000081885 | MassIVE | Thu Jan 04 17:11:00 GMT 2018

SECONDARY ACCESSION(S): PXD008597

REPOSITORIES: MassIVE

Json Xml
altmetric image

Publications

Convergent evolution provides insights into the selective drivers underlying evolutionary change. Snake venoms, with a direct genetic basis and clearly defined functional phenotype, provide a model system for exploring the repeated evolution of adaptations. While snakes use venom primarily for predation, and venom composition often reflects diet specificity, three lineages of cobras have independently evolved the ability to spit venom at adversaries. Using gene, protein, and functional analyses,  ...[more]

Similar Datasets

Proteomics Compositional analysis of venom in three species of Indian cobra (Naja) and their cross-reactivity with Indian polyvalent Antivenom
2023-05-10 | PXD027361 | Pride
Proteomics Venom and saliva proteomics of Solenodon
2018-04-24 | MSV000082307 | MassIVE
Proteomics Top-down venom proteomics of Walterinnesia aegyptia and Walterinnesia morgani
2021-01-15 | MSV000086709 | MassIVE
Metabolomics GNPS Top-down venomics of 12 turkish viper species
2022-04-04 | MSV000089193 | GNPS
Proteomics Venom gland transcriptome and venom proteome of the Tibellus oblongus spider unveil molecular diversity of polypeptide toxins
2023-10-23 | PXD040219 | Pride
Proteomics DATASET - Top-Down - Snake venom proteomics of seven taxa of the genera Vipera, Montivipera, Macrovipera and Daboia across Turkiye/Turkey
2024-03-04 | MSV000094229 | MassIVE
Proteomics Top-down Proteomics of Venom Gland Organoids
2024-03-07 | MSV000094268 | MassIVE
Proteomics Top-down Proteomics of Venom Gland Organoids II
2024-03-07 | MSV000094269 | MassIVE
Proteomics Top-down venomics of the East African green mamba and the black mamba
2016-03-24 | MSV000079607 | MassIVE
Other Top-down venomics of 12 turkish viper species
2022-04-04 | MSV000089193 | MassIVE