Project description:Samples from mice infected and then treated with vehicle, carnitine or benznidazole in the chronic stage of infection. Tissue samples extracted with 50% methanol followed by 3:1 dichloromethane:methanol. C8 chromatography with negative mode data acquisition
Project description:Large intestine samples from mice infected with 50,000 Trypanosoma cruzi parasites or left uninfected. One week post-infection, mice were treated with carnitine, benznidazole or vehicle. Animals were euthanized after 10 days of treatment and organs collected. Metabolites were extracted with 50% methanol followed by 3:1 dichloromethane-methanol and analyzed by C8 chromatography, with positive mode ddMS2 data collection (data-dependent).
Project description:Male C3H/HeJ mice were infected with 50,000 luciferase-expressing strain CL Brener trypanosoma cruzi parasites. Beginning 7 days post-infection, mice were treated with carnitine in drinking water (100 mg/kg/day dosing equivalent), benznidazole (100 mg/kg/day by intraperitoneal injection), or left untreated (vehicle group). One additional control group was uninfected. Mice were euthanized and liver and oesophagus collected at day 17 post-infection. Tissue was extracted with 50% methanol followed by 3:1 dichloromethane:methanol, and analyzed by C8 chromatography, with detection by MS in positive mode.
Project description:Large intestine samples from mice infected with 50,000 Trypanosoma cruzi parasites or left uninfected. One week post-infection, mice were treated with carnitine, benznidazole or vehicle. Animals were euthanized after 10 days of treatment and organs collected. Metabolites were extracted with 50% methanol followed by 3:1 dichloromethane-methanol and analyzed by C8 chromatography, with negative mode ddMS2 data collection (data-dependent).
Project description:Immunoglobulin diversity includes B-cell receptor, T-cell receptor, and antibody diversity. Existing studies focus more on the role of B-cell and T-cell receptor diversity in tumor immunity, while little is known about antibody diversity. This study examined and compared the blood exosomes of lung cancer patients and healthy individuals via proteomics and bioinformatics analyses. The results showed that of the 270 identified proteins, those enriched in the defense mechanism items were the most abundant. Most were antibody subtype molecules, accounting for 50.00%. Similarly, of the 40 identified exosomal DEPs, 29 were enriched in the defense mechanism items (72.50%), with a higher proportion of antibody subtypes (82.76%). Furthermore, 24 DEP antibody molecules were involved in 18 immune reaction-related signaling pathways. These results indicated that human serum exosomes contained a large number of antibody molecules, while the antibody subtypes from the lung cancer serum exosomes differed from those of the healthy controls. The variation in antibody diversity may be closely related to LA tumor immunity.
Project description:Samples from mice infected and then treated with vehicle, carnitine or benznidazole in the chronic stage of infection. Tissue samples extracted with 50% methanol followed by 3:1 dichloromethane:methanol. C8 chromatography with negative mode data acquisition