ABSTRACT: A comparative global natural products social (GNPS) molecular networking analysis of 63 co-isolated fungi guided the isolation of new scopularides C-G from Beauveria sp. CMB-F585
Project description:A comparative global natural products social (GNPS) molecular networking analysis of 63 co-isolated fungi guided the isolation of new scopularide H and known scopularide A from Scopulariopsis sp. CMB-F115
Project description:A comparative global natural products social (GNPS) molecular networking analysis of 63 co-isolated fungi guided isolation of new scopularides C-H and the known scopularides A and B from Scopulariopsis spp. CMB-F458 and CMB-F115, and Beauveria sp. CMB-F585
Project description:Chemical investigation of Scopulariopsis sp. CMB-F458 yielded the known scopularides A and B, coupled with a comparative global natural products social (GNPS) molecular networking analysis of 63 co-isolated fungi guided the isolation of six new scopularides C-H from Beauveria sp. CMB-F585 and Scopulariopsis sp. CMB-F115
Project description:Chemical investigations of Penicillium sp. CMB-STF067 is based on both the antibacterial property of its extract and the Global Natural Product Social (GNPS) molecular networking analysis of 176 soil-associated fungi, guided the isolation of 4-new xanthoquinodins, jugiones A-D.
Project description:Social experience influences multiple behaviors of many animal species, including aggression. Social isolation often increases aggressiveness. To investigater the molecular basis of social influences on aggressiveness, we performed comparative gene expression profiling on heads from 6-day-old, single-housed, more aggressive and group-housed, less aggressive male flies. Keywords: social experience
Project description:Social isolation poses a severe mental and physiological burden on humans. Most animal models that investigate this effect are based on prolonged isolation, which does not mimic the milder conditions experienced by people in the real world. Here we show that in the medial amygdala, a brain structure that is crucial for social memory, acute social isolation causes social memory loss and significant changes in specific mRNAs and proteins.
Project description:Gene expression profiling was carried out on peripheral blood leukocytes from 14 healthy older adults. The primary research question is whether gene expression differs in individuals experiencing chronically high levels of social isolation (by UCLA Loneliness Scale) vs chronically low levels of social isolation. Experiment Overall Design: Gene expression profiling was carried out on peripheral blood leukocytes from 14 healthy older adults. The primary research question is whether gene expression differs in individuals experiencing chronically high levels of social isolation (by UCLA Loneliness Scale) vs chronically low levels of social isolation.
Project description:In this study, we use mass spectrometry (MS)-based proteomics to define the host response under single and dual infection states of macrophages with C. neoformans and K. pneumoniae to define critical responses of each biological system. This high-resolution comparative analysis illustrates how protein abundance deviates during a transition from an acute to chronic infectious state with exposure to additional microbial stimuli. We reveal global changes upon infection followed by pathogen-specific host response signatures. Additionally, we define regulatory changes within C. neoformans as the fungi adapts to the host environment and stabilizes prior to further disruption in the presence of chronic bacterial infection. We validate our findings with host cytokine detection and phenotypic profiling of the fungi throughout the host and bacterial exposures. Overall, our study provides an in-depth analysis of cross-kingdom protein level changes during macrophage infection. This information provides new insight into fungal modulation of the immune response, including a stabilization and adaptation of the host and fungi during a chronic infection, which is disrupted upon chronic co-infection with a bacterial pathogen.
Project description:We used comparative proteomic approach to identify proteome changes in purified synaptic terminals (synaptosomes) of rats exposed to chronic social isolation (CSIS) (6 weeks), animal model for depression, followed by chronic Fluoxetine treatment (last 3 weeks of CSIS) (therapeutic effect). Our aim was to examine a subcellular compartment enriched in proteins involved in synaptic function.