Metabolomics

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GNPS - R_OM_Metapyo_32_CF_patients_C18_pos

ABSTRACT: we analyzed the metabolome content by untargeted HPLC/HRMS (see supplementary information). Isolates were first grown in synthetic CF medium 2 (SCFM2), a culture medium designed to mimick the nutritional conditions in CF pulmonary mucus (Turner et al. 2014). Intracellular metabolic extracts of mid-log cultures were harvested by fast-filtration and mechanical lysis, and the contents were normalized to a constant colony-forming units over optical density at 595 nm ratio (CFU/DO), as described by Aros-Calt et al. (Aros-Calt et al. 2019). Metabolomics analysis were performed using two complementary HPLC/HRMS methods to optimize chemical coverage, and data were processed and normalized following the most up-to-date methods

INSTRUMENT(S): Q Exactive

ORGANISM(S): Pseudomonas Aeruginosa (ncbitaxon:287)

SUBMITTER: audrey LE GOUELLEC  

PROVIDER: MSV000084790 | GNPS | Fri Jan 10 12:18:00 GMT 2020

REPOSITORIES: GNPS

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<i>Pseudomonas aeruginosa</i> (<i>P.a</i>) is one of the most critical antibiotic resistant bacteria in the world and is the most prevalent pathogen in cystic fibrosis (CF), causing chronic lung infections that are considered one of the major causes of mortality in CF patients. Although several studies have contributed to understanding <i>P.a</i> within-host adaptive evolution at a genomic level, it is still difficult to establish direct relationships between the observed mutations, expression o  ...[more]

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