Project description:A pull-down assay by incubating the His-tagged PPARalpha-LBD with various brain tissue (cortex, cerebellum, and hippocampus) extracts.
Project description:The Ts1Cje mouse model of Down syndrome (DS) has partial triplication of mouse chromosome 16 (MMU16), which is partially homologous to human chromosome 21. The mouse model develops various neuropathological features identified in DS individuals. We analysed the effect of partial triplication of the MMU16 segment on global gene expression in the cerebral cortex, cerebellum and hippocampus of Ts1Cje mice at 4 time-points; postnatal day (P)1, P15, P30 and P84. RNA was extracted from thre brain regions (Cerebral cortex, hippocampus and cerebellum) for hybridization to arrays from 3 pairs of Ts1Cje and disomic C57BL/6 littermate control for each timepoints at postnatal (P) day 1, P15, P30 and P84.
Project description:<p><em>Bacteroides thetaiotaomicron</em> (B. theta) dominates the gut microbiome of most mammals. This strictly anaerobic gut symbiont colonizes the mucus layer of host intestinal epithelial cells in both healthy and diseased conditions. Reduced neuronal and vagal afferent innervation observed in germ-free mice was found to be normalized by colonialization with B. theta. In addition to deficits in gut innervation, germ-free mice have been reported to have reduced neuronal number and neurotransmitter levels in the brain. Here, we investigated the hallmarks of Alzheimer’s disease (AD) in the brain of germ-free mice compared to mice mono-colonized with B. theta. We analysed the number of mature neurons, neurotransmitter transporters, amyloid precursor protein processing, and inflammatory status in three brain regions: the hippocampus, prefrontal cortex (PFC) and cerebellum. The hippocampus and the prefrontal cortex are regions thought to be highly susceptible to pathogenesis whereas the cerebellum is thought to be only mildly affected. Interestingly, secretion of neuroprotective APPsa decreased in hippocampus and remained unchanged in PFC, while levels were increased in the cerebellum in response to bacterial colonization. In addition, the number of presynaptic boutons increased in the hippocampus but remained unaffected in the cerebellum.</p>
Project description:Cell surface N-glycomic analysis of select functional mouse brain areas including forebrain, hindbrain, cortex, hippocampus, and cerebellum.
Project description:4 samples from 9 brain regions Brain tissue from the New South Wales Tissue Resource Centre, 9 brain regions, 4 samples each: 1 male alcoholic, 1 female alcoholic, 1 male control, 1 female control. Brain regions: pre-frontal cortex, cerebral cortex, visual cortex, thalamus, hippocampus, amygdala, caudate nucleus, putamen, cerebellum
Project description:Rheumatoid arthritis (RA) is linked to depression and dementia in later life by inflammatory involvement of the central nervous system (CNS). Regional heterogeneity of brain immunophenotypes was described under homeostasis, but a topographical resolution of CNS immune responses in chronic peripheral inflammatory diseases like RA is missing. We demonstrate regional heterogeneity of CNS susceptibility to chronic peripheral inflammation in the human tumor necrosis factor α transgenic (TNFtg) mouse model of RA. TNFtg mice showed myeloid cell infiltration, microglial activation, and a mutual transcriptomic fingerprint of neuroinflammation in the cortex, striatum, and thalamus. Immune responses were minimal in the hippocampus and cerebellum. We demonstrate regional CNS immune responses to chronic peripheral inflammation, sparing the hippocampus and cerebellum and reversible by peripheral anti-inflammatory treatment. Targeting microenvironmental susceptibility or resilience of brain regions will help to prevent and treat RA-related neuropsychiatric comorbidity. RNA-sequencing was performed from five brain regions (cortex, striatum, thalamus, hippocampus, and cerebellum) from C57Bl6/J wild type mice and TNFtg mice (strain Tg197; kindly provided by George Kollias (Fleming Institute, Vari, Greece).
Project description:We aim to analyse high-throughput data deriving from miRNAs expression profiles to thoroughly investigate the miRNAs changes in the four brain regions of adult rats including cerebellum, hippocampus, hypothalamus and cortex
Project description:We aim to analyse high-throughput data deriving from gene expression profiles to thoroughly investigate the transcriptomic changes in the four brain regions of adult rats including cerebellum, hippocampus, hypothalamus and cortex.
Project description:Survey of gene expression in ten common inbred strains of laboratory mouse. Seven brain regions examined: amygdala, basal ganglia, cerebellum, frontal cortex, hippocampus, cingulate cortex, olfactory bulb. Keywords: Genetic background and brain region Sample data tables were removed because the ID_REF identifiers did not match the platform IDs
Project description:To establish the consequences of whole-animal deletion of CYP2E1, we isolated RNA from dissected brain tissue (cerebellum, medial prefrontal cortex, dorsal hippocampus, and ventral hippocampus) of 16-week male and female WT and KO mice. Mice were maintained on a normal chow diet and were euthanized using CO2 and decapitation prior to brain dissection.