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GNPS A global lipid map reveals host dependency factors conserved across SARS-CoV-2 variants

ABSTRACT: Using non-targeted LC-MS/MS lipidomics approaches, we profiled SARS-CoV-2 changes in cellular lipid composition for both 293T HEK infected cells and cells ectopically expressing individual SARS-CoV-2 proteins, in total examining 26 experimental conditions and three control conditions, each in biological quintuplicate.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Jennifer Kyle

PROVIDER: MSV000087944 | GNPS | Wed Aug 04 19:20:00 BST 2021

REPOSITORIES: GNPS

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A global lipid map reveals host dependency factors conserved across SARS-CoV-2 variants.

Farley Scotland E SE Kyle Jennifer E JE Leier Hans C HC Bramer Lisa M LM Weinstein Jules J Bates Timothy A TA Lee Joon-Yong JY Metz Thomas O TO Schultz Carsten C Tafesse Fikadu G FG

bioRxiv : the preprint server for biology 20220215

A comprehensive understanding of host dependency factors for SARS-CoV-2 remains elusive. We mapped alterations in host lipids following SARS-CoV-2 infection using nontargeted lipidomics. We found that SARS-CoV-2 rewires host lipid metabolism, altering 409 lipid species up to 64-fold relative to controls. We correlated these changes with viral protein activity by transfecting human cells with each viral protein and performing lipidomics. We found that lipid droplet plasticity is a key feature of ...[more]

PMID: 35194611

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