Project description:Bacillus strains grown in LB media. Metabolite extraction from the cells was performed using 100% methanol at different growth stages.
Project description:Transcriptomic analysis of Bacillus subtilis wild-type strain and hfq mutant in stationary phase of growth using to tiling array gene expression analysis. RNA-binding protein Hfq is a key component of the adaptive responses of many proteobacterial species. In these organisms, the importance of Hfq largely stems from its participation to regulatory mechanisms involving small non-coding RNAs. In contrast, the function of Hfq in Gram-positive bacteria has remained elusive. 97 transcription units (representing 134 genes) were found significantly different between the wild-type and the ΔhfqBs strains in the stationary cultures performed in rich LB medium.
Project description:we investigated three Bacillus strains (HT1, HT2, and HT3) isolated from the soybean root microbiome for their potential plant growth-promoting and biocontrol activities. Bacillus-HT1 and HT2 significantly enhanced soybean seed germination, while Bacillus-HT3 promoted leaf area expansion, indicating strain-specific developmental effects. To elucidate the molecular basis of these effects, we conducted shotgun proteomic profiling of soybean leaves. The analysis revealed significant modulation of proteins involved in key biological processes, including amino acid metabolism, biosynthesis of cellular nitrogen and aromatic compounds, and cellular component organization and biogenesis. Notably, proteins such as anthranilate synthase and proteasome subunit alpha type were differentially expressed, suggesting the activation of growth- and defense-related pathways. These findings provide mechanistic insights into how specific Bacillus strains modulate soybean development at the molecular level and highlight their potential for use as bio-inoculants to enhance crop productivity and resilience under stress conditions.
Project description:Transcriptomic analysis of Bacillus subtilis wild-type strain and hfq mutant in stationary phase of growth using to tiling array gene expression analysis. RNA-binding protein Hfq is a key component of the adaptive responses of many proteobacterial species. In these organisms, the importance of Hfq largely stems from its participation to regulatory mechanisms involving small non-coding RNAs. In contrast, the function of Hfq in Gram-positive bacteria has remained elusive. 97 transcription units (representing 134 genes) were found significantly different between the wild-type and the ?hfqBs strains in the stationary cultures performed in rich LB medium. This data set contains 4 samples. Expression profiles of Bacillus subtilis prototype strain (BSB1, a tryptophan-prototrophic derivative 168 strain) and a ?hfq mutant were examined 5 h after the onset of stationary phase in LB medium. Two biological replicates were analyzed.
Project description:This project contributes to the proteomic comparison of Bacillus cereus ATCC 14579 wild-type strain without its pBClin15 plasmid, its mutant strain devoided of the methionine sulfoxide reductase AB (msrAB) protein and the corresponding msrAB complemented strain, all strains grown in aerobiosis condition and harvested at three growth stages.
Project description:This project contributes to the proteomic comparison of Bacillus cereus ATCC 14579 wild-type strain without its pBClin15 plasmid, its mutant strain devoided of the methionine sulfoxide reductase AB (msrAB) protein and the corresponding msrAB complemented strain, all strains grown in aerobiosis condition and harvested at three growth stages.
Project description:Comparing the transcriptional responses of Bacillus subtilis strains WN624 and WN1106 at 5 kPa and 101 kPa. WN1106 is a 5 kPa-evolved strain with increased fitness compared to ancestor-WN624 strain at 5 kPa. This experiment probed the difference in response when the strains are grown at 5 kPa.
Project description:In this study two genome-reduced Bacillus subtilis strains lacking about 36% of dispensable genetic information were constructed using a markerless and scarless deletion method. In order to analyze the consequences of the deletions for the bacteria, a multi-omics characterization of the reference strain Δ6 (Westers et al., 2003; PMID 12949151) and the two deletion strains was carried out. Bacteria were cultivated in complex medium supplemented with glucose, and samples of the same cultures were subjected to metabolome, proteome, and transcriptome analyses.These revealed a massive re-organization of metabolism as well as substantial changes in the transcriptome and the proteome.
Project description:To uncover the effects of KrrA regulation on gene transcription and define the bacterial response imposed by this regulation, a transcriptomic study was carried out in which Bacillus anthracis krrA was compared to WT in two growth conditions: LB in the presence or absence of ‘205.