Project description:Converted from profile raw to centroid mzML data with ThermoRawFileParser executable through macOS:
Algae bloom simulation Lab 8 for the course Untargeted Metabolomics Summer School 2022
Project description:Background Vaccinia virus (VACV) infection induces prominent changes in host cell metabolism. Little is known about the global metabolic reprogramming that takes place in the whole tissue during viral infection. Here, we performed an unbiased longitudinal metabolomics study in VACV-infected mice to investigate metabolic changes in the tissue during infection. We assessed metabolites in homogenized skin over time in the presence or absence of antigen-specific T cells using untargeted mass spectrometry. VACV infection induced several significant metabolic changes, including in the levels of nucleic acid metabolites (reflecting the impact of viral replication on the skin metabolome). Furthermore, monocyte- and antiviral T cell-produced metabolites, including itaconic acid, glutamine, and glutathione, were significantly increased following infection, highlighting the immune response’s contribution to the global skin metabolome. Additional RNA-Seq of infected skin tissue recapitulated transcriptional changes identified via metabolomics. Overall, our study reveals the metabolic balance of viral replication and the antiviral immune response in the skin and identifies metabolic pathways that could contribute to cutaneous poxvirus control in vivo.
Project description:Pulmonary exposure to multiwalled carbon nanotubes (MWCNT) induces an inflammatory and rapid fibrotic response, although the long-term signaling mechanisms are unknown. The aim of this study was to perform genome-wide mRNA profiling in mice blood to identify non-invasive blood based biomarkers for medical and occupational surveillance. The pathological results in this 1-year MWCNT post-exposure study was previously published in Snyder-Talkington et al. J Toxicol Environ Health A. 2016;79(8):352-66. doi: 10.1080/15287394.2016.1159635. Epub 2016 Apr 19. PMID: 27092743
Project description:Untargeted metabolomics data generated for the above publication for 13 lowland willows in Czech Republic and Austria. Extraction and methods are reported in Leong et al. (Accepted) and Sedio et al. 2021 Frontiers in Ecology and Evolution, https://doi.org/10.3389/fevo.2021.679638 [doi:10.25345/C59Z90N8R] [dataset license: CC0 1.0 Universal (CC0 1.0)].
Project description:Converted from profile raw to centroid mzML data with ThermoRawFileParser executable through macOS:
Algae bloom simulation Lab 8 for the course Untargeted Metabolomics Summer School 2022
Project description:Metabolomics data of extracts from Orbicella faveolata field samples. Corals are healthy and affected by SCTLD. Samples from ongoing study (summer and fall, multiple sites).
Project description:Human iPSCs and NSCs were engineered by AAVS1 and/or C13 safe-harbor TALENs which mediated targeted integration of various reporter genes at single or dual safe-harbor loci. Multiple clones of targeted human iPSCs were used to compare with parental untargeted NCRM5 iPSCs. Polyclonal targeted human NSCs were used to compare with their parental untargeted NCRM1NSCs or H9NSCs. Total RNA obtained from targeted human iPSCs or NSCs compared to untargeted control iPSCs or NSCs.