Project description:Converted from profile raw to centroid mzML data with ThermoRawFileParser executable through macOS:
Algae bloom simulation Lab 8 for the course Untargeted Metabolomics Summer School 2022
Project description:Converted from profile raw to centroid mzML data with ThermoRawFileParser executable through macOS:
Algae bloom simulation Lab 8 for the course Untargeted Metabolomics Summer School 2022
Project description:Subset of MassIVE MSV000085944 data published by Panitchpakdi and collaborators (2022) used during Metabolomics Summer School 2023 at Statens Serum Institut, Copenhagen.
Panitchpakdi M, Weldon KC, Jarmusch AK, Gentry EC, Choi A, Sepulveda Y, et al. (2022) Non-invasive skin sampling detects systemically administered drugs in humans. PLoS ONE 17(7): e0271794. https://doi.org/10.1371/journal.pone.0271794
Project description:Raw metaproteomic data of Chinese indoor dust derived extracellular vesicles. These dust samples were collected in the summer of 2022 and were immediately sent to the laboratory for processing. The extracellular vesicles of dust were subjected to ultracentrifugation, and they were further purified using iodixanol density gradient ultracentrifugation. After extracting the extracellular vesicles, metaproteomic sequencing was performed.
Project description:study of diphenhydramine in blood and skin - untargeted mass spectrometry analysis [doi:10.25345/C5SJ2X] - reupload for summer school
Project description:To examine the difference of the endometrial conditions in the cows between on summer and autumn seasons, gene expression profiles were compared. The expressions of 268 genes were significantly higher in the endometrium collected on summer than those on autumn, whereas 369 genes were lower (P<0.05 or lower). Transcripts of GP2 and NTS were more abundant in the endometrium of summer than those of autumn (P<0.05). In contrast, the mRNA expressions of CDH1 and HSPH1 were lower in the endometrium of summer seasons (P<0.1). Collectively, the different gene expression profiles may contribute to functional differences of endometrium between the summer and autumn seasons, and GP2 and NTS may have a relevance to endometrial deficiency that cause infertility of cows on summer seasons.
Project description:We used a DNA microarray chip covering 369 resistance types to investigate the relation of antibiotic resistance gene diversity with humans’ age. Metagenomic DNA from fecal samples of 123 healthy volunteers of four different age groups, i.e. pre-school Children (CH), School Children (SC), High School Students (HSS) and Adults (AD) were used for hybridization. The results showed that 80 different gene types were recovered from the 123 individuals gut microbiota, among which 25 were present in CH, 37 in SC, 58 in HSS and 72 in AD. Further analysis indicated that antibiotic resistance genes in groups of CH, SC and AD can be independently clustered, and those ones in group HSS are more divergent. The detailed analysis of antibiotic resistance genes in human gut is further described in the paper DNA microarray analysis reveals the antibiotic resistance gene diversity in human gut microbiota is age-related submitted to Sentific Reports
Project description:Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment and pathogens. Heredity appears to be a major factor determining the sensitivity of oysters to summer mortality, allowing resistant (R) and susceptible (S) lines to be produced. We conducted genome-wide expression profiling of R and S gonads during the 3-month period preceding a summer mortality event using a 9K cDNA microarray that we designed. This transcriptional analysis provides new indications to define markers for Quantitative Trait Loci searches and functional studies, and evaluates the potential role of each gene in the resistance to summer mortality