ABSTRACT: This dataset consists of plasma, gut and fat tissue from patients with Crohn's disease with data acquired on Orbitrap in positive ionization mode.
Project description:In Crohn's disease, creeping fat is the characteristic expansion of mesenteric adipose tissue wrapping around the inflamed intestine. Through a comparative transcriptomic analysis of creeping fat and normal-looking mesenteric adipose tissues from patients with Crohn's disease and non-Crohn's disease, we found that a dynamic transcriptional and cell compositional change occurs during the progression from non-Crohn's disease to Crohn's disease, and finally to creeping fat.
Project description:This dataset consists of plasma from patients with NASH disease and healthy age- and BMI- matched healthy volunteers with data acquired on Orbitrap in positive ionization mode.
Project description:This dataset consists of plasma from patients with NASH disease and healthy age- and BMI- matched healthy volunteers with data acquired on Orbitrap in positive ionization mode.
Project description:This project study and comprehensively characterize the lysine acetyltion in the human gut microbiome using antibody-based enrichment strategry and Orbitrap mass spectrometer. The technique has also been applied to study the microbiome in pediatric Crohn's disease and control subjects in order to understand the functional alterations of microbiome in IBD.
Project description:Our hypothesis is that in IBD patients intestinal bacteria translocation into the intra-abdominal fat depots affects adipocyte morphology and gene expression. The study aimed to study adipocyte gene expression of omental (OM) and mesenteric (MES) adipose tissue of ulcerative colitis (UC) and crohn's disease (CD).
Project description:The aim of this study was to characterize the transcriptional signature of MDR1+ human memory T cells isolated from clinically inflamed gut tissue, and compare it to local MDR1- memory T cells Human mononuclear cells were isolated from the peripheral blood of a healthy adult donor (Ficol density centrifugation) or from resected lesioned gut tissue of a patient with active Crohn's disease. For cell isolation from gut tissue, tissue was rinsed with PBS, treated with 0.15% DTT to remove mucous, then with 1 mM EDTA to remove epithelial cells and intra-epithelial lymphocytes. Remaining tissue was digested using Liberase-TL (Roche) plus 10 U/mL DNase I. Mononuclear cells were then filtered through 70 mM nylon filters and isolated via a 30%/ 70% Percol gradient. Mononuclear cells from blood or gut tissue were FACS-sorted into CD3+ CD4+ CD45RO+ MDR1+ or MDR1- memory T cells, using Rhodamine 123 (Rh123) efflux as a surrogate for MDR1 expression/ activity. Sorted cells were harvested directly ex vivo (without further in vitro culture or manipulation) prior to RNA extraction. MDR1- memory CD4+ T cells vs. MDR1+ memory CD4+ T cells from healthy donor peripheral blood or from active Crohn's disease lesioned tissue; MDR1- or MDR1+ memory CD4+ T cells from blood vs. inflamed gut tissue
Project description:Our hypothesis is that in IBD patients intestinal bacteria translocation into the intra-abdominal fat depots affects adipocyte morphology and gene expression. The study aimed to study adipocyte gene expression of omental (OM) and mesenteric (MES) adipose tissue of ulcerative colitis (UC) and crohn's disease (CD). Total RNA was extracted from isolated adipocytes from omental and mesenteric adipose tissue of CD and UC patients. Microarray experiments were performed in duplicates of 4 different pools of RNAs extracted from adipocytes isolated from OM and MES of UC patients (n=5) and CD patients (n=5) respectively.
Project description:Longitudinal metaproteomics data set looking at the gut microbiome metaproteomes of fivepost-surgery Crohn's Disease patients for five time points over the course of a year.
Project description:Lysine acetylation is a rich post-translational modification in prokaryotes that regulates multiple immune response pathways, and its importance has been overlooked. Crohn's disease is a subtype of inflammatory bowel disease characterized by chronic inflammation involving the entire gastrointestinal tract. CD-induced intestinal damage and subsequent disability is a growing global health problem. However, the pathophysiology of this disease is not fully understood, and the level of protein acetylation in Crohn's gut tissue is not well understood.To determine the level of protein acetylation in different intestinal sites of CD and changes in intestinal immune response after colonic protein acetylation. Colon, cecum, and rectal tissues of Crohn's disease and healthy control mice were detected by acetylation proteomics. ResultsThe analysis determined that there were up to 624 acetylated proteins and 1809 acetylation sites in intestinal tissue, which were different in the disease and control groups, and the acetylated proteins were enriched in immunomodulatory signaling pathways such as NF-κB, MAPK, and Wnt.