Project description:‘Candidatus Liberibacter asiaticus’ (CLas), the bacterial pathogen associated with citrus greening disease, is transmitted by Diaphorina citri, the Asian citrus psyllid (ACP). Percoll gradient density centrifugation was used to fractionate an ACP homogenate to generate a sample enriched for intact microbial cells (CLas and insect endosymbionts) and associated ACP cells. Proteins were extracted from Percoll gradient fractions prepared in triplicate from CLas(-) ACP samples and CLas(+) ACP samples.
Project description:Lisbon lemon trees were grafted with budwood infected with the citrus greening bacterium, 'Candidatus Liberibacter asiaticus', or control budwood, and leaf samples were collected every two weeks post graft for LC/MS analysis. This project has shotgun proteomics data for leaf samples from 5 control and 5 CLas grafted trees at the 10 week post graft timepoint.
Project description:Lisbon lemon trees were grafted with budwood infected with the citrus greening bacterium, 'Candidatus Liberibacter asiaticus', or control budwood, and leaf samples were collected every two weeks post graft for LC/MS analysis. This project has shotgun proteomics data for leaf samples from 5 control and 5 CLas grafted trees at the 14 week post graft timepoint.
Project description:Lisbon lemon trees were grafted with budwood infected with the citrus greening bacterium, 'Candidatus Liberibacter asiaticus', or control budwood, and leaf samples were collected every two weeks post graft for LC/MS analysis. This project has shotgun proteomics data for leaf samples from 5 control and 5 CLas grafted trees at the two week post graft timepoint.
Project description:We evaluated the effect on citrus trees of two newly-identified molecules, benzbromarone and tolfenamic acid, used as antimicrobials in commercial groves of sweet orange (Citrus sinensis). We delivered the molecules by trunk injection and evaluated safety and efficacy parameters by performing RNAseq of the citrus host responses.