Project description:‘Candidatus Liberibacter asiaticus’ (CLas), the bacterial pathogen associated with citrus greening disease, is transmitted by Diaphorina citri, the Asian citrus psyllid (ACP). Percoll gradient density centrifugation was used to fractionate an ACP homogenate to generate a sample enriched for intact microbial cells (CLas and insect endosymbionts) and associated ACP cells. Proteins were extracted from Percoll gradient fractions prepared in triplicate from CLas(-) ACP samples and CLas(+) ACP samples.
Project description:This dataset contains several sponge-derived bacteria, along with data from the sponges to enable the assignation of the biosynthetic source of detected metabolite features. Media controls and solvent blanks are also included. Please see metadata for the bacterial source sponge.
Project description:Some soil bacteria promote plant growth, including Pseudomonas species. With this approach we detected significant changes in Arabidopsis genes related to primary metabolism that were induced by the bacteria.
Project description:Huanglongbing (HLB) is a devastating disease of citrus plants caused by the non-culturable phloem-inhabiting bacterium Candidatus Liberibacter asiaticus (CLas). Bacteria are vectored by the psyllid Diaphorina citri and introduced into sieve cells, evading plant immunity and establishing a successful infection in citrus plants. Although no resistance has been reported in Citrus species, complete or partial resistance has been documented in the distant relatives Murraya paniculate and Bergera koenigii, providing excellent systems to investigate molecular mechanisms leading to either resistance or susceptibility. The first weeks after bacterial release into the phloem are critical for the establishment of the bacteria. In this study, a thorough transcriptomic analysis of young flushes exposed to CLas-positive or negative psyllid has been performed in Citrus x sinensis, as well as in these resistant species along the first eight weeks after exposure. Our results suggest that the resistant genotypes do not deploy a classical immunity response. Instead, transcriptome changes are scarce and only a few genes are differentially expressed. Functional analysis suggest that primary metabolism and other basic cellular functions could be rewired in the resistant genotypes to limit infection. Transcriptomes of young flushes of the three species are very different, supporting the existence of distinct biochemical landscapes. These findings suggest that both intrinsic metabolic inadequacies to CLas survival, as well as inducible reprogramming of physiological functions upon CLas recognition, could orchestrate together to restrict bacteria multiplication in these resistant hosts.
Project description:Evaluation of different strategies to interpret metaproteomics data acquired on soil samples from a floodplain along the Seine River (France) incorporating sample-specific metagenomics data, soil genome catalogue database, and generic sequence database.
2022-02-17 | PXD026798 | Pride
Project description:Soil bacteria and archaea along soil profile
Project description:This dataset contains several sponge-derived bacteria, along with data from the sponges to enable the assignation of the biosynthetic source of detected metabolite features. Media controls and solvent blanks are also included. Please see metadata for the bacterial source sponge.
Project description:Phosphorus (P) limitation will play a key role in the productivity of agriculture in the coming decades. Struvite is an ammonium magnesium phosphate mineral that can be recovered from wastewater-treatment plants and can be considered as an alternative source of P. However, the impact of struvite on the plant yield and, particularly, on the soil microbial community is barely known. Here, we tested the impacts of struvite, sewage sludge, and their combination on the barley yield, soil macro and micronutrients, and biochemical and microbiological soil properties. Amendment with struvite alone and its combination with sludge increased the availability of P in soil, the plant uptake of P and Mg, and the barley yield. The analysis of phospholipid fatty acids (PLFAs) and metaproteomics approaches revealed significant effects of struvite on the biomass of Gram-positive bacteria and, particularly, on actinobacterial populations in soil.