Project description:Transcriptomic analysis of fungus Penicillium decumbens and brlA deletion strains in liquid medium and solid medium respectivelly Examination of differential gene expressions by Penicillium decumbens strains 114-2 and brlA deletion stains in liquid medium and solid medium
Project description:We performed integrated targeted lipidomics and untargeted metabolomics on patients' plasma (n = 81) from MPXV without HIV (MPLWOH), MPXV with HIV (MPLWH), HIV-positive without MPXV (PLWH) and healthy. Exosome proteomics and cytokine profiling complemented lipidome/metabolome analyses for functional interpretation.
Project description:Brevicompanines are natural products isolated from the culture filtrate of the fungus Penicillium brevicompactum. They showed plant growth regulating properties in several species including lettuce, rice or Arabidopsis thaliana. We used microarrays to gather information about the reprogramming of gene transcription when Arabidopsis leaves were treated with Brevicompanine C (BrvC) that showed significant activity in plant growth assays.
Project description:Full clinical data for a cohort of 199 individuals with acute coronary syndrome.
Untargeted serum metabolomics using the Metabolon platform for individuals with ACS (n=156).
Serum metabolomics using the Nightingale Health (NMR) platform for individuals with ACS and controls (ACS, n=191; controls, n=961).
Project description:We discovered, through untargeted metabolomics, that creatine promotes ferroptosis in colorectal cancer. Our study identifies ERK2 as a creatine sensor that mediates active FSP1 and ferroptosis resistance, and highlights the potential of targeting tumor SLC6A8 for cancer treatment.
Project description:<p>The Chinese caterpillar fungus, Ophiocordyceps sinensis, is a valuable medicinal entomopathogenic fungus, but artificial cultivation is hindered by fruiting body abortion, severely impacting yield and quality. The interactions between the fungus, its host insects, and soil are critical for its development. Multi-omics analyses were conducted to compare normally and abnormally developing samples, examining microbial communities and metabolites in endophytic larvae and the surrounding mycosphere soil. Abnormal stroma development significantly altered microbial ecology. It increased fungal diversity in both larvae and soil, while reducing bacterial diversity within larvae. The microbial composition shifted markedly, characterized by a high enrichment of Penicillium fungi and a depletion of Bacillus bacteria in and around abnormal samples. Cross-kingdom microbial network analysis in these samples showed fewer connections and lower stability. Metabolomic profiling revealed significant enrichment of the antibiotics N1-hydroxy-roquefortine C and glandicoline A, both derivatives of roquefortine C, alongside the enrichment of multiple antibiotic biosynthesis pathways. Partial Least Squares Structural Equation Modelling indicated that Penicillium toxicity likely induces microbial dysbiosis, and this disruption of microbial homeostasis is the primary cause of fruiting body abortion. The findings suggest that Penicillium overgrowth is detrimental to normal stroma formation. Conversely, Bacillus may play a crucial role in suppressing Penicillium, offering a potential target for biocontrol strategies. This study is the first to elucidate the key role of Penicillium-mediated toxicity and microbial imbalance in the developmental disorders of Chinese Cordyceps.</p>