In-depth proteomic analysis of mouse microglia using a combination of FASP and StageTip-based, high pH, reversed-phase fractionation.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD000168. Experiment: BV_2_High_pH_biological_1, file: 20121208_L_4.mzML. Published as part of Proteomics. 2013 Aug 14 . From the Abstract: {{i}} ... A characterization of microglia proteome would facilitate on the study of microglial functions in association with various neurodegenerative diseases. To build a reference proteome, we established a BV-2 microglial proteome to a depth of 5494 unique protein groups using a novel strategy that combined FASP, StageTip-based high pH fractionation, and high-resolution mass spectrometry quickly and cost-efficiently. By bioinformatics analysis, the BV-2 proteome is a valuable resource for studies of microglial function, such as in the immune response, inflammatory response, and phagocytosis ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Mouse, Abelson_murine_leukemia_virus, Ectromelia_virus, Mouse_mammary_tumor_virus, Mouse_parvovirus_1, Mouse_parvovirus_2, Mouse_parvovirus_3, Mouse_parvovirus_4a, Mouse_parvovirus_5a, Murid_herpesvirus_1, Murid_herpesvirus_2, Murid_herpesvirus_4, Murine_adenovirus_3, Murine_adenovirus_a, Murine_hepatitis_virus_a59, Murine_hepatitis_virus_jhm, Murine_norovirus_1, Murine_osteosarcoma_virus, Murine_pneumotropic_virus, Murine_polyomavirus, Murine_type_c_retrovirus

DISEASE(S): Not Available

SUBMITTER: Dohyun Han, et al.  

PROVIDER: GPM11210021378 | GPMDB |

REPOSITORIES: GPMDB