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Omics score: 1019

Compartment resolved reference proteome map from highly purified na-ve, activated, effector, and memory CD8+ murine immune cells.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD001065. Experiment: Aus7, file: Aus7_35.mzml. Published as part of Proteomics. 2015 Jan 23 . From the Abstract: {{i}} In this study, we investigated the proteome of four cytotoxic T cell subtypes; na-ve, recently activated effector, effector and memory cells. Cells were fractionated into membrane, cytosol, soluble nuclear, chromatin-bound and cytoskeletal compartments. Following LC-MS/MS analysis, identified peptides were analyzed via MaxQuant. Compartment fractionation and gel-LC-MS separation resulted in 2399 proteins identified in total. {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Mus_musculus_viruses, Mouse

DISEASE(S): Not Available

SUBMITTER: Zanker D, et al.

PROVIDER: GPM32310006565 | GPMDB |

REPOSITORIES: GPMDB

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Compartment resolved reference proteome map from highly purified naïve, activated, effector, and memory CD8⁺ murine immune cells.

Zanker Damien D Otto Wolfgang W Chen Weisan W von Bergen Martin M Tomm Janina M JM

Proteomics 20150303 11

Differentiation of CD8(+) T lymphocytes into effector and memory cells is key for an adequate immune response and relies on complex interplay of pathways that convey signals from the cell surface to the nucleus. In this study, we investigated the proteome of four cytotoxic T-cell subtypes; naïve, recently activated effector, effector, and memory cells. Cells were fractionated into membrane, cytosol, soluble nuclear, chromatin-bound, and cytoskeletal compartments. Following LC-MS/MS analysis, ide ...[more]

PMID: 25643623

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