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Identification of targets of the protein disulfide reductase thioredoxin

ABSTRACT: Identification of targets of the protein disulfide reductase thioredoxin using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and thiol specific differential labeling with isotope-coded affinity tags (ICAT). Reduction of specific target disulfides is quantified by measuring ratios of cysteine residues labeled with the heavy (13C) and light (12C) ICAT reagents in peptides derived from tryptic digests of Trx-treated and non-treated samples. Keywords: protein, LC-MS/MS, ICAT

ORGANISM(S): Hordeum vulgare

PROVIDER: GSE11650 | GEO | 2008/11/20

SECONDARY ACCESSION(S): PRJNA106161

REPOSITORIES: GEO

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