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Omics score: 1009

Quantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeast.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD001019. Experiment: Dis14, file: folder summary. Published as part of Elife. 2014 Jul 29:e03023 . From the Abstract: {{i}} Aneuploidy causes severe developmental defects and is a near universal feature of tumor cells. Despite its profound effects, the cellular processes affected by aneuploidy are not well characterized. Here, we examined the consequences of aneuploidy on the proteome of aneuploid budding yeast strains. We show that although protein levels largely scale with gene copy number, subunits of multi-protein complexes are notable exceptions. Posttranslational mechanisms attenuate their expression when their encoding genes are in excess. Our proteomic analyses further revealed a novel aneuploidy-associated protein expression signature characteristic of altered metabolism and redox homeostasis ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Yeast

DISEASE(S): Not Available

SUBMITTER: Dephoure N, et al.

PROVIDER: GPM32320002303 | GPMDB |

REPOSITORIES: GPMDB

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Quantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeast.

Dephoure Noah N Hwang Sunyoung S O'Sullivan Ciara C Dodgson Stacie E SE Gygi Steven P SP Amon Angelika A Torres Eduardo M EM

eLife 20140729

Aneuploidy causes severe developmental defects and is a near universal feature of tumor cells. Despite its profound effects, the cellular processes affected by aneuploidy are not well characterized. Here, we examined the consequences of aneuploidy on the proteome of aneuploid budding yeast strains. We show that although protein levels largely scale with gene copy number, subunits of multi-protein complexes are notable exceptions. Posttranslational mechanisms attenuate their expression when their ...[more]

PMID: 25073701

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Quantitative Proteomic Analysis Reveals Posttranslational Responses to Aneuploidy in Yeast

Project description:Aneuploidy causes severe developmental defects and is a near universal feature of tumor cells. Despite its profound effects, the cellular processes affected by aneuploidy are not well characterized. Here, we examined the consequences of aneuploidy on the proteome of aneuploid budding yeast strains. We show that although protein levels largely scale with gene copy number, subunits of multi-protein complexes are notable exceptions. Posttranslational mechanisms attenuate their expression when their encoding genes are in excess. Our proteomic analyses further revealed a novel aneuploidy-associated protein expression signature characteristic of altered metabolism and redox homeostasis. Indeed aneuploid cells harbor increased levels of reactive oxygen species (ROS). Interestingly, increased protein turnover attenuates ROS levels and this novel aneuploidy-associated signature and improves the fitness of most aneuploid strains. Our results show that aneuploidy causes alterations in metabolism and redox homeostasis. Cells respond to these alterations through both transcriptional and posttranscriptional mechanisms.

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