Browse
Submit Data
Databases
API
Help

Dataset Information

12 Views

218 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 1004

Alterations in the interactome of serine/threonine protein phosphatase type-1 in atrial fibrillation patients.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD000697. Exiperiment: PP1_Mouse_raw_IgG, file: OR8_121211_NL_DC_PP1_Gel4_B_labelfree2.mzml. Published as part of J Am Coll Cardiol. 2015 Jan 20;65(2):163-73 . From the Abstract: {{i}} Cardiac lysates were coimmunoprecipitated with anti-PP1c antibody followed by mass spectrometry-based, quantitative profiling of associated R-subunits. Subsequently, label-free quantification (LFQ) was used to evaluate altered R-subunit-PP1c interactions in PAF patients. R-subunits with altered binding to PP1c in PAF were further studied using bioinformatics, Western blotting (WB), immunocytochemistry, and coimmunoprecipitation. {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Mus_musculus_viruses, Mouse

DISEASE(S): Not Available

SUBMITTER: Chiang DY, et al.

PROVIDER: GPM32320014437 | GPMDB |

REPOSITORIES: GPMDB

ACCESS DATA

Json Xml

Publications

Alterations in the interactome of serine/threonine protein phosphatase type-1 in atrial fibrillation patients.

Chiang David Y DY Lebesgue Nicolas N Beavers David L DL Alsina Katherina M KM Damen J Mirjam A JM Voigt Niels N Dobrev Dobromir D Wehrens Xander H T XH Scholten Arjen A

Journal of the American College of Cardiology 20150101 2

<h4>Background</h4>Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia, yet current pharmacological treatments are limited. Serine/threonine protein phosphatase type-1 (PP1), a major phosphatase in the heart, consists of a catalytic subunit (PP1c) and a large set of regulatory (R)-subunits that confer localization and substrate specificity to the holoenzyme. Previous studies suggest that PP1 is dysregulated in AF, but the mechanisms are unknown.<h4>Objectives</h4>The purpose ...[more]

PMID: 25593058

Similar Datasets

Alterations in the Interactome of Serine/Threonine Protein Phosphatase Type-1 in Atrial Fibrillation Patients

Project description:Objectives: To test the hypothesis that serine/threonine protein phosphatase type-1 (PP1) is dysregulated in paroxysmal atrial fibrillation (pAF) at the level of its regulatory subunits (R-subunits). Background: AF is the most common sustained cardiac arrhythmia yet current pharmacologic treatment is ineffective. PP1, a major phosphatase in the heart, consists of a catalytic subunit (PP1c) and a large set of R-subunits that confer localization and substrate specificity to the holoenzyme. Previous studies suggest that PP1 is dysregulated in AF but the mechanisms are unknown. Methods: Cardiac lysates were co-immunoprecipitated with anti-PP1c antibody followed by mass spectrometry-based (quantitative) profiling of associated R-subunits. Subsequently, label-free quantification was used to evaluate altered R-subunit-PP1c interactions in pAF patients. R-subunits with altered binding to PP1c in pAF were further validated using qRT-PCR, Western blotting (WB), immunocytochemistry, and co-immunoprecipitation. Results: 135 and 78 putative PP1c-interactors were captured respectively from mouse ventricles and human atria, with many previously unreported interactors with conserved PP1c-docking motifs. Increases in binding were found between PP1c and PPP1R7, CSDA, and PDE5A in pAF patients, with CSDA and PDE5A being novel interactors validated by bioinformatics, immunocytochemistry and co-immunoprecipitation. WB confirmed that these upregulated associations cannot be ascribed to changes in global protein expression alone. Conclusion: Subcellular heterogeneity in PP1 activity and downstream protein phosphorylation in AF may be attributed to alterations in PP1c-R-subunits interactions, which impair PP1 targeting to proteins involved in electrical and Ca2+-remodeling. This represents a novel concept in AF pathogenesis and provides highly-specific drug targets for treating AF.

2015-03-30 | PXD000697 | Pride

Analysis of Circulating Breast Cancer Cell Heterogeneity and Interactions with Peripheral Blood Mononuclear Cells.

Project description:Purpose: To infer interactions between circulating breast cancer cells and peripheral mononuclear cells using bioinformatics analysis of single cell RNA-sequencing. Methods: Filter based method for capturing single cells from 7.5mL of blood from patients with breast cancer, followed by single cell RNA-sequencing. Results: Transcriptomes predicted for two CTC populations with distinct expression profiles and interactions with peripheral blood mononuclear cells.

2020-08-25 | GSE139495 | GEO

Effect of embryo lysates treatment on small RNA expression

Project description:To systematically evaluate the effect of embryo lysates feeding, we conducted small RNA sequencing on day 7 N2 worms treated with either embryo or day 6 worm lysates. Our findings revealed that embryo lysates affected the 21ur-6059 expression.

2024-09-07 | GSE265889 | GEO

Effect of embryo lysates treatment on gene expression.

Project description:To systematically evaluate the effect of embryo lysates feeding, we conducted RNA sequencing (RNA-seq) on day 7 N2 worms treated with embryo lysates. Our findings revealed that embryo lysates affected the expression of genes related to fatty acid metabolism, degradation of branched-chain amino acids, and lysosome functions. Notably, worms treated with embryo lysates showed increased fatty acid degradation gene expression and decreased fatty acid elongation gene expression.

2024-09-07 | GSE265886 | GEO

Allergen induced gene expression of airway epithelial cells shows a possible role for TNF-α

Project description:Response to allergen was studied in bronchial epithelial cell line H292. Cells were cultured and subsequently exposed to House dust mite or vessel (saline) Microarray data was analysed using bioinformatics and biostastics. We find a strong response to allergen in epithelial cells Keywords: cellular response to allergen

2007-10-01 | GSE9151 | GEO

Blood transcription profiling of infections of humans with Leishmania infantum.

Project description:Gene expression profiling to address the effects of infection with Leishmania infantum during distinct clinical outcomes as active visceral leishmaniasis (VL), remission of disease and asymptomatic infection. Total RNA was extracted from whole-blood lysates obtained from VL patients, treated VL patients, asymptomatic individuals and uninfected controls. The aim was to evaluate gene expression signatures associated with protective and pathological responses.

2016-10-25 | E-GEOD-77528 | biostudies-arrayexpress

Interactome analysis of PYHIN immune regulators

Project description:The human PYHIN proteins, AIM2, IFI16, IFIX, and MNDA, are regulators of immune response, transcription, apoptosis, and cell cycle. However, their protein interactions and underlying mechanisms remain largely uncharacterized. Here, we provide the interaction network for all PYHIN proteins. By designing a cell-based inducible system and integrating microscopy, immunoaffinity capture, quantitative mass spectrometry, and bioinformatics, we identified over 300 PYHIN interactions reflective of diverse functions.

2016-07-20 | MSV000079955 | MassIVE

Analysis of differential expression profiles of Long noncoding RNA in peripheral blood of patients with active rheumatoid arthritis

Project description:we studied the expression profile of lncRNA in peripheral blood of patients with active RA LncRNA and mRNA expression in peripheral blood from two activity RA patients and two healthy donors were analyzed with microarray profiling. peripheral blood of five active RA patients and five healthy control members were collected. Quantitative real-time PCR was further used to screen and verify the expression of lncRNA to verify the expression of the selected lncRNA; perform bioinformatics analysis to study the potential function of the differentially expressed genes, construct a receiver operating characteristic (ROC) curve to evaluate the clinical diagnostic value of the identified lncRNA, and use correlation analysis to explore the relationship between aberrantly expressed lncRNA that may have diagnostic value and clinical indicators.

2020-06-10 | GSE134087 | GEO

T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination - 2010

Project description:Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people. Total RNA was extracted from whole-blood lysates obtained from individuals immediately prior to vaccination against seasonal influenza, followed by depletion of globin messenger RNA. The goal was to evaluate potential gene expression signatures at Day 0 associated with immune senescence.

2014-07-20 | E-GEOD-58898 | biostudies-arrayexpress

T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination - 2009

2014-07-20 | E-GEOD-58943 | biostudies-arrayexpress