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Omics score: 1003

Proteomics of Aggregatibacter actinomycetemcomitans Outer Membrane Vesicles.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD002509. File: 2013_10_16_Prep_3_Serotype_e_third_experiment_combined_mgf.mgf. Published as part of PLoS One. 2015 Sep 18;10(9):e0138591 . From the Abstract: {{i}} ... To improve our understanding of the pathogenicity-associated functions that A. actinomycetemcomitans exports via OMVs, we studied the proteome of density gradient-purified OMVs from a rough-colony type clinical isolate, strain 173 (serotype e) using liquid chromatography-tandem mass spectrometry (LC-MS/MS) ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Aggregatibacter_actinomycetemcomitans_anh9381, Aggregatibacter_actinomycetemcomitans_d11s_1

DISEASE(S): Not Available

SUBMITTER: Kieselbach T

PROVIDER: GPM32320018111 | GPMDB |

REPOSITORIES: GPMDB

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Proteomics of Aggregatibacter actinomycetemcomitans Outer Membrane Vesicles.

Kieselbach Thomas T Zijnge Vincent V Granström Elisabeth E Oscarsson Jan J

PloS one 20150918 9

Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive forms of periodontitis and with endocarditis. Outer membrane vesicles (OMVs) released by this species have been demonstrated to deliver effector proteins such as cytolethal distending toxin (CDT) and leukotoxin (LtxA) into human host cells and to act as triggers of innate immunity upon carriage of NOD1- and NOD2-active pathogen-associated molecular patterns (PAMPs). To improve our understanding of t ...[more]

PMID: 26381655

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Project description:Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive forms of periodontitis, and with endocarditis. Outer membrane vesicles (OMVs) released by this species have been demonstrated to deliver effector proteins such as cytolethal distending toxin (CDT) and leukotoxin (LtxA) into human host cells, and to act as triggers of innate immunity upon carriage of NOD1- and NOD2-active pathogen-associated molecular patterns (PAMPs). To improve our understanding of the pathogenicity-associated functions that A. actinomycetemcomitans exports via OMVs, we have used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to characterize the OMV-associated proteome of a rough-colony type clinical isolate, strain 173 (serotype e). This yielded identification of 151 proteins overlapping in three out of four independent experiments, using density gradient-purified OMVs. Further to confirming the vesicle-associated release of LtxA, and of several proteins earlier demonstrated to act as immunoreactive antigens in the human host, we identified numerous additional putative virulence-related proteins in the A. actinomycetemcomitans OMV proteome, including proteins involved in immune evasion, drug targeting, and iron/nutrient acquisition. In summary our findings are consistent with an OMV-associated proteome that exhibits several offensive and defensive functions, and they provide a comprehensive basis to further disclose roles of A. actinomycetemcomitans OMVs in periodontal and systemic disease.

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