Dataset Information

Quantitative proteomic analysis of Ricinus communis during early seed imbibition provided novel insights into cold stress response

ABSTRACT: Early planting is one of the strategies used to increase grain yield in temperate regions. However, poor cold tolerance in castor inhibits seed germination, resulting in lower seedling emergence and biomass. To date, proteomics studies have identified diverse proteins associated with germinating or developing castor seeds; however, the regulators involved in early imbibed castor seeds under cold stress are rare. Here, the elite castor variety Tongbi 5 was used to identify the differential abundance protein species (DAPS) between cold stress (4 °C) and control conditions (30 °C ) imbibed seeds. As a result, 127 proteins were identified to be differentially accumulated according to iTRAQ strategy. Bioinformatics analyses revealed that 84 DAPS were annotated in 24 GO functional groups, 70 DAPS were classified into 16 COG categories, and 56 DAPS were involved in 16 KEGG pathways. Based on physiological index assays and iTRAQ results, this study provided some invaluable insights into the cold stress responses of early imbibed castor seeds.

ORGANISM(S): Ricinus Communis

SUBMITTER: Xiaoyu Wang

PROVIDER: PXD010043 | iProX | Tue Jun 05 00:00:00 BST 2018

REPOSITORIES: iProX

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Publications

Quantitative Proteomic Analysis of Castor (<i>Ricinus communis</i> L.) Seeds During Early Imbibition Provided Novel Insights into Cold Stress Response.

Wang Xiaoyu X Li Min M Liu Xuming X Zhang Lixue L Duan Qiong Q Zhang Jixing J

International journal of molecular sciences 20190116 2

Early planting is one of the strategies used to increase grain yield in temperate regions. However, poor cold tolerance in castor inhibits seed germination, resulting in lower seedling emergence and biomass. Here, the elite castor variety Tongbi 5 was used to identify the differential abundance protein species (DAPS) between cold stress (4 °C) and control conditions (30 °C) imbibed seeds. As a result, 127 DAPS were identified according to isobaric tag for relative and absolute quantification (iT ...[more]

PMID: 30654474

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Project description:To better undersand the effects of drought stress on wheat developing seeds, the transcription profile of early developing wheat seeds under control and drought stress conditions were comparatively analyzed by using the Affymetrix wheat geneChip. Drought stress is a major yield-limiting factor for wheat. Wheat yields are particularly sensitive to drought stress during reproductive development. Early seed development stage is an important determinant of seed size, one of the yield components. We specifically examined the impact of drought stress imposed during postzygotic early seed development in wheat. We imposed a short-term drought stress on plants with day-old seeds and observed that even a short-duration drought stress significantly reduced the size of developing seeds as well as mature seeds. Drought stress delayed the developmental transition from syncytial to cellularized stage of endosperm. Coincident with reduced seed size and delayed endosperm development, a subset of genes associated with cytoskeleton organization was misregulated in developing seeds under drought-stressed. Several genes linked to hormone pathways were also differentially regulated in response to drought stress in early seeds. Notably, drought stress strongly repressed the expression of wheat storage protein genes such as gliadins, glutenins and avenins as early as 3 days after pollination. Our results provide new insights on how some of the early seed developmental events are impacted by water stress, and the underlying molecular pathways that can possibly impact both grain size and quality in wheat. Winter wheat cultivar Redland, PI 502907 (Triticum aestivum L.) was used for this study. Seedlings were vernalized at 4°C for 6 weeks and then transplanted to a one gallon pot of soil-sand mixture (3:1, v/v) and grown in a growth chamber under the following conditions: relative humidity, 50–70%; 16-h light/8-h dark photoperiod; 21°C daytime temperature and 18°C nights. Plants were watered regularly twice daily at the rate of 100ml/ per pot. Because, wheat has an asynchronous fertilization pattern for ovlues in the inflorescence, each floret needs to be specifically marked for timing the fertilization and stress induction. After spikes developed, unfertilized ovules were monitored and observed for the fertilization process. Closed wheat spikes with anthers outside were marked as fertilized. Drought stress was imposed 24h after the fertilization (HAF). Drought stress treatment was initiated by discontinuing watering on the drought treatment plants while control plants were regularly watered twice daily. Stress treatment was applied at 48 HAF and relieved at 96 HAF. The microarray study focuses on 24 HAF to 72 HAF in control and drought stress conditions. We started to impose drought stress 24HAF.

Dataset Information

Quantitative proteomic analysis of Ricinus communis during early seed imbibition provided novel insights into cold stress response

Publications

Quantitative Proteomic Analysis of Castor (<i>Ricinus communis</i> L.) Seeds During Early Imbibition Provided Novel Insights into Cold Stress Response.

Similar Datasets

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