A multiplex fragment ion-based method for accurate proteome quantification
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens Escherichia Coli
SUBMITTER: 
Lihua Zhang
PROVIDER: PXD012457 | iProX | Tue Jan 22 00:00:00 GMT 2019
REPOSITORIES: iProX
Publications
A Multiplex Fragment-Ion-Based Method for Accurate Proteome Quantification.
Liu Jianhui J Zhou Yuan Y Shan Yichu Y Zhao Baofeng B Hu Yechen Y Sui Zhigang Z Liang Zhen Z Zhang Lihua L Zhang Yukui Y
Analytical chemistry 20190301 6
Multiplex proteome quantification with high accuracy is urgently required to achieve a comprehensive understanding of dynamic cellular and physiological processes. Among the existing quantification strategies, fragment-ion-based methods can provide highly accurate results, but the multiplex capacity is limited to 3-plex. Herein, we developed a multiplex pseudo-isobaric dimethyl labeling (m-pIDL) method to extend the capacity of the fragment-ion-based method to 6-plex by one-step dimethyl labelin ...[more]
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