Proteomics

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Additional precursor purification in isobaric mass tagging experiments by traveling wave ion mobility separation (TWIMS).


ABSTRACT: We investigated if additional peptide purification by traveling wave ion mobility separation (TWIMS) can reduce precursor contamination using a mixture of S. cerevisiae and HeLa proteomes. In accordance with previous reports on FAIMS-Orbitrap instruments, we find that TWIMS provides a remarkable improvement (on average 2.85 times) in signal to noise ratio for sequence ions. We also report, that TWIMS reduces reporter ions contamination by around one third (to 14 - 15% contamination) and even further (to 6-9%) when combined with narrowed quadrupole isolation window.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Pavel Shliaha  

LAB HEAD: Kathryn Susan Lilley

PROVIDER: PXD001047 | Pride | 2014-06-10

REPOSITORIES: Pride

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Publications

Additional precursor purification in isobaric mass tagging experiments by traveling wave ion mobility separation (TWIMS).

Shliaha Pavel V PV   Jukes-Jones Rebekah R   Christoforou Andy A   Fox Jonathan J   Hughes Chris C   Langridge James J   Cain Kelvin K   Lilley Kathryn S KS  

Journal of proteome research 20140616 7


Despite the increasing popularity of data-independent acquisition workflows, data-dependent acquisition (DDA) is still the prevalent method of LC-MS-based proteomics. DDA is the basis of isobaric mass tagging technique, a powerful MS2 quantification strategy that allows coanalysis of up to 10 proteomics samples. A well-documented limitation of DDA, however, is precursor coselection, whereby a target peptide is coisolated with other ions for fragmentation. Here, we investigated if additional pept  ...[more]

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