Ion mobility tandem mass spectrometry enhances performance of bottom-up proteomics

ABSTRACT: We describe a novel method utilizing ion mobility-based concentration of peptide fragment ions on a qTOF mass spectrometer improving the sensitivity of bottom-up proteomics by up to 10-fold. This enabled the identification of 7,500 human proteins within one day and 8,600 phosphorylation sites within 5h of LC-MS/MS time. The method also proved powerful for multiplexed quantification experiments exemplified by the chemoproteomic interaction analysis of HDACs with Trichostatin A.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Spleen, Testis, Ovary, Prostate Gland, Lung, Liver, Kidney

SUBMITTER: Hannes Hahne  

LAB HEAD: Bernhard Kuster

PROVIDER: PXD000863 | Pride | 2015-08-18

REPOSITORIES: Pride