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Analysis of Human Membrane Protein Complexes by Using Formaldehyde Cross-linking and Protein Correlation Profiling

ABSTRACT: Membrane proteins are essential to many biological processes and account for two-thirds of the successful drug targets. Studying the membrane-protein complexes assembly will help to understand their biological functions comprehensively. We applied an approach that combines cross-linking mass spectrometry and protein correlation profiling to analysis the endogenous human membrane protein complexes. Finally, we constructed a network of 312 complexes involves 31,095 high-confident interactions with 2,333 proteins. These protein complexes are linked to core biological processes such as translation, intracellular transport, metabolic, and catabolic. They are also related to more than one hundred of human diseases, especially Alzheimer's disease, Carcinoma, and Endocarditis. Strikingly, 939 proteins participate in the interaction between different organelles, including ER and mitochondria, plasma membrane and mitochondria, and ER and vesicles. Besides that, possible cross-linking peptides were obtained, which provide further evidence and detailed structural information for the predicted protein complexes. This membrane-protein associated complexes map might benefit disease research and provide a new perspective on organelle communication.

ORGANISM(S): Homo Sapiens

SUBMITTER: Cuihong Wan

PROVIDER: PXD013998 | iProX | Fri May 24 00:00:00 BST 2019

REPOSITORIES: iProX

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