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A model dataset for quantitative cross-linking/mass spectrometry using isotope-labeled cross-linkers

ABSTRACT: Dynamic proteins and multi-protein complexes govern most biological processes. Cross-linking/mass spectrometry (CLMS) is increasingly successful in providing residue-resolution data on static proteinaceous structures. In order to investigate the technical feasibility of recording dynamic processes using isotope-labelling for quantitation, we generated a model dataset by cross-linking human serum albumin (HSA) with the readily available cross-linker BS3-d0/d4 in different heavy/light ratios.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Zhuo Chen

LAB HEAD: Juri Rappsilber

PROVIDER: PXD004107 | Pride | 2016-05-06

REPOSITORIES: Pride

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Quantitative cross-linking/mass spectrometry using isotope-labelled cross-linkers.

Fischer Lutz L Chen Zhuo Angel ZA Rappsilber Juri J

Journal of proteomics 20130326

Dynamic proteins and multi-protein complexes govern most biological processes. Cross-linking/mass spectrometry (CLMS) is increasingly successful in providing residue-resolution data on static proteinaceous structures. Here we investigate the technical feasibility of recording dynamic processes using isotope-labelling for quantitation. We cross-linked human serum albumin (HSA) with the readily available cross-linker BS3-d0/4 in different heavy/light ratios. We found two limitations. First, isotop ...[more]

PMID: 23541715

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Quantitative cross-linking/mass spectrometry

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