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Clamping down on transcription: repression of archaeal RNAP by the novel viral regulator RIP

ABSTRACT: Little is known about how archaeal viruses perturbate the transcription machinery of their hosts. We provide the first example of a non-DNA binding archaeo-viral transcription factor that directly targets the host RNA polymerase and efficiently represses its activity. We have used cross-linking and mass spectrometry to identify how ORF145 binds in the DNA-binding channel of RNA polymerase.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Sulfolobus Solfataricus

SUBMITTER: Adam Belsom

LAB HEAD: Juri Rappsilber

PROVIDER: PXD003282 | Pride | 2016-12-02

REPOSITORIES: Pride

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Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP.

Sheppard Carol C Blombach Fabian F Belsom Adam A Schulz Sarah S Daviter Tina T Smollett Katherine K Mahieu Emilie E Erdmann Susanne S Tinnefeld Philip P Garrett Roger R Grohmann Dina D Rappsilber Juri J Werner Finn F

Nature communications 20161124

Little is known about how archaeal viruses perturb the transcription machinery of their hosts. Here we provide the first example of an archaeo-viral transcription factor that directly targets the host RNA polymerase (RNAP) and efficiently represses its activity. ORF145 from the temperate Acidianus two-tailed virus (ATV) forms a high-affinity complex with RNAP by binding inside the DNA-binding channel where it locks the flexible RNAP clamp in one position. This counteracts the formation of transc ...[more]

PMID: 27882920

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