Aurora B kinase disrupts the extended conformation of lattice-bound Kinesin-13 MCAK

ABSTRACT: We reveal that MCAK has a compact conformation in solution using cross-linking and electron microscopy. When MCAK is bound to the microtubule ends, it adopts an extended conformation with the N terminus and neck region of MCAK interacting with the microtubule. Also Aurora Bphosphorylation does not alter MCAK conformation in solution. Aurora B interferes with the extended conformation of MCAK on microtubules to decrease the affinity of MCAK for microtubules and reduces its depolymerase activity in a graded fashion.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Juan Zou  

LAB HEAD: Prof. Juri Rappsilber

PROVIDER: PXD008215 | Pride | 2019-01-07

REPOSITORIES: Pride