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Crosslinking mass spectrometry of the unconventional kinetoplastid kinetochore protein KKT4

ABSTRACT: The kinetochore is the macromolecular protein machine that drives chromosome segregation by interacting with spindle microtubules. Unlike most other eukaryotes that have canonical kinetochore proteins, a group of evolutionarily divergent eukaryotes called kinetoplastids (such as Trypanosoma brucei that causes human African trypanosomiasis) have a unique set of kinetochore proteins. To date, the only kinetoplastid kinetochore protein that is known to bind microtubules is KKT4, which has no high-resolution structure information available. Here we used X-ray crystallography, NMR spectroscopy, and crosslinking mass spectrometry to characterise the structure and dynamics of KKT4.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Trypanosoma Brucei

SUBMITTER: Bungo Akiyoshi

LAB HEAD: Bungo Akiyoshi

PROVIDER: PXD020229 | Pride | 2020-10-15

REPOSITORIES: Pride

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