Protein Structure Determination in Biological Matrices by Mass Spectrometry and Computational Biology

ABSTRACT: We have implemented the use of a heterobifunctional, UV photoactivatable cross-linker, which greatly increases the number of identified cross-links compared with homobifunctional, NHS-ester based cross-linkers. We have cross-linked human serum albumin in the context of human blood serum. We present a novel methodology that combines the use of this high-resolution cross-linking with conformational space search to investigate the structure of proteins in their native environment.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Adam Belsom  

LAB HEAD: Juri Rappsilber

PROVIDER: PXD001692 | Pride | 2015-09-22

REPOSITORIES: Pride